Abstract
Brasenia schreberi J.F. Gmelin is a declared endangered species found in the lakes and ponds of South Korea. For planning its conservation strategy, we examined the genetic diversity within and among six populations, using randomly amplified polymorphic DNA (RAPD) and amplified fragment length polymorphism (AFLP). Polymorphisms were more frequently detected per loci with AFLP (69.3%) than RAPD (36.8%). High genetic diversity was recognized within populations: polymorphic loci (PPL) values ranged from 36.3% in the CJM population to 74.5% in the GGT population, with a mean value of 47.8% based on AFLP markers. Great genetic differentiation (θB) was detected among the six populations (0.670 on RAPD and 0.196 on AFLP), and we calculated a low rate of gene flow (Nem), i.e., 0.116 on RAPD and 0.977 on AFLP. Furthermore, a Mantel test revealed that no correlation existed between genetic distances and geographical distances among the six local populations, based on RAPD or AFLP markers. These results are attributed to a number of factors, including an insufficient length of time for genetic diversity to be reduced following a natural decline in population size and isolation, adaptation of the genetic system to small population conditions, and a restricted gene flow rate. Based on both its genetic diversity and population structure, we suggest that a strategy for conserving and restoringB. schreberi must focus on maintaining historical processes, such as high levels of outbreeding, while monitoring increased gene flow among populations. This is because a reduction in genetic diversity as a result of genetic drift is undesirable.
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Literature cited
Archak S, Gaiwad AB, Gautam D, Rao EVVB, Swamy KRM, Karihaloo JL (2003) Comparative assessment of DNA fingerprinting techniques (RAPD, ISSR, and AFLP) for genetic analysis of cashew (Anacardium occidentale L.) accessions of India. Genome 46: 362–369
Aston, HI (1977) Aquatic Plants of Australia. Melbourne University Press, Australia, pp 46–48
Bertin RI (1993) Incidence of monoecy and dichogamy in relation to self-fertilization in angiosperms. Amer J Bot 80: 557–560
Chen D-H, Ronald PC (1999) A rapid DNA minipreparation method suitable for AFLP and other PCR applications. Plant Mol Biol Rep 17: 53–57
Chen J, Gituru WR, Liu X, Wang Q (2007) Genetic diversity inIsoetes yunguiensis, a rare and endangered endemic fern in China. Front Biol China 2: 46–49
Chen Y-Y, Li X-L, Yin L-Y, Li W (2008) Genetic diversity of the threatened aquatic plantOttelia alismoides in Yangtze River. Aquat Bot 88: 10–16
Choi H-K (2007) Cabombaceae Rich ex A Rich,In C-w Park et al., eds, The Genera of Vascular Plants of Korea. Academy Publishing Co. Press, Seoul, p 163
Chrysler MA (1938) The winter buds ofBrasenia. Bull Torrey Bot Club 65: 277–283
Dong Y-H, Chen J-M, Gituru RW, Wang Q-F (2007) Gene flow in populations of the endangered aquatic fernCeratopteris pteridoides in China as revealed by ISSR. Aquat Bot 87: 69–74
Elakovich SD, Wooten JW (1987) An examination of the phytotoxicity of the water shield,Brasenia schreberi. J Chem Ecol 13: 1935–1940
Excoffier L (1993) Analysis of Molecular Variance (AMOVA), ver. 1.55. Genetics and Biometry Laboratory, University of Geneva, Switzerland
Excoffier L, Smouse PE, Quattro JM (1992) Analysis of molecular variation inferred from metric distances among DNA haplotypes: Application to human mitochondrial DNA restriction data. Genetics 131: 479–491
Fischer M, Husi R, Prati D, Peintinger M, Kleunen M, Schmid B (2000) RAPD variation among and within small and large populations of the rare clonal plantRanunculus reptans (Ranunculaceae). Amer J Bot 87: 1128–1137
Griffin SR, Mavrganis K, Eckert CG (2000) Experimental analysis of protogyny inAquilegia canadensis (Ranunculaceae). Amer J Bot 87: 1246–1256
Hartl DL, Clark AG (1997) Principles of Population Genetics, 3rd ed. Sinauer Associates, Sunderland, MA, USA
Holsinger KE, Lewis PO (2005) Hickory: A Package for Analysis of Population Genetic Data, ver. 1.0.4. Department of Ecology and Evolutionary Biology, University of Connecticut, Storrs, CT, USA
Holsinger KE, Lewis PO, Dey DK (2002) A Bayesian approach to inferring population structure from dominant markers. Mol Ecol 11: 1157–1164
Kang M, Ye Q, Huang H (2005) Genetic consequence of restricted habitat and population decline in endangeredIsoetes sinensis (Isoetaceae). Ann Bot-London 96: 1265–1274
Keiper FJ, McConchie R (2000) An analysis of genetic variation in natural populations ofSticherus flabellatus [R. Br. (St John)] using amplified fragment length polymorphism (AFLP) markers. Mol Ecol 9: 571–581
Kim C, Na HR, Choi H-K (2008) Genetic diversity and population structure of endangeredIsoetes coreana in South Korea based on RAPD analysis. Aquat Bot 89: 43–49
Kim Y-D (1996) Characterization of water and sediment environment in water shield (Brasenia schreberi) habitats. Kor J Ecol 19:209–216
Koga K, Kadono Y, Setoguchi H (2007) The genetic structure of populations of the vulnerable aquatic macrophyteRanunculus nipponicus (Ranunculaceae). J Plant Res 120: 167–174
Lee H-W, Choung H-L, Roh T-H, Kwon Y-H, Kim CH, Hyun J-O, Chang IS (2005) Categorization and Conservation of the Threatened Plant Species in Environmental Impact Assessment. Korea Environment Institute, Seoul
Lee NS, Yean SH, Park JO, Roh MS (2006) Molecular evidence for hybridization ofIlex ×wandonensis (Aquifoliaceae) by RAPD analysis. J Plant Biol 49: 491–497
Lee S, Ma S, Lim Y, Choi H-K, Shin H (2004) Genetic diversity and its implications in the conservation of endangeredZostera japonica in Korea. J Plant Biol 47: 275–281
Lesica R Leary RF, Allendorf FW, Bilderback DE (1988) Lack of genetic diversity within and among populations of an endangered plant,Howellia aquatilis. Conserv Biol 2: 275–282
Li W, Xia L-Q, Li J-Q, Wang G-X (2004) Genetic diversity ofPotamogeton maackianus in the Yangtze River. Aquat Bot 80: 227–240
Liu R Yang YS, Hao CY, Guo WD (2007) Ecological risk assessment using RAPD and distribution pattern of a rare and endangered species. Chemosphere 68: 1497–1505
Liu Y, Wang Y, Huang H (2006) High interpopulation genetic differentiation and unidirectional linear migration patterns inMyricaria laxiflora (Tamaricaceae), and endemic riparian plant in the Three Gorges Valley of the Yangtze River. Amer J Bot 93: 206–215.
Lopez-Pujol J, Font J, Simon J, Blanche C (2007) Can the preservation of historical relicts permit the conservation of endangered plant species? The case of Silenesennenii (Caryophyllaceae). Conserv Genet 8: 903–912
Loveless MD, Hamrick JL (1984) Ecological determinants of genetic structure in populations. Annu Rev Ecol Syst 15: 65–95
Mantel N (1967) The detection of disease clustering and a generalized regression approach. Cancer Res 27: 209–220
Miller MP (1997) Tools for Population Genetics Analysis (TFGPA). A Windows Program for the Analysis of Allozyme and Molecular Population Genetic Data, ver. 1.3. Department of Biological Sciences, Northern Arizona University, Flagstaff, AZ, USA
Miller MP (1998) AMOVA-PREP. A Program for the Preparation of Input Files from Dominant-marker Row Data, ver. 1.01. Department of Biological Sciences, Northern Arizona University, Flagstaff, AZ, USA
Nei M (1978) Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics 89: 583–590
Oh MJ, Na H-R, Choi H-K, Liu JR, Kim SW (2008) High frequency plant regeneration from zygotic-embryo-derived embryogenic cell suspension cultures of watershield (Brasenia schreberi). Plant Biotechnol Rep 2: 87–92
Osborn JM, Schneider EL (1988) Morphological studies of the Nymphaceae sensulato. XVI. The floral biology ofBrasenia schreberi. Ann Mo Bot Gard 75: 778–794
Palacios C, Gonzalez-Candelas F (1999) AFLP analysis of the critically endangeredLimonium cavanillesii (Plumbaginaceae). J Hered 90: 485–489
Perez T, Albornoz J, Dominguez A (1998) An evaluation of RAPD fragment reproducibility and nature. Mol Ecol 7: 1347–1357
Prentis PJ, Mather PB (2008) Fine-scale patterns of genetic variation indicate non-equilibrium gene frequency divergence in the stream lily,Helmholtzia glaberrima. Freshwater Biol 53: 973–980
Raymond M, Dansereau P (1953) The geographical distribution of the bipolar Nymphaceae,Nymphaea tetragona, andBrasenia schreberi. Proc Pacific Sci Congr 5: 122–131
Rossetto M, Weaver PK, Dixon KW (1995) Use of RAPD analysis in devising conservation strategies for the rare and endangeredGrevillea scapigera (Proteaceae). Mol Ecol 4: 357–364
SAS Institute Inc (1999) SAS Proprietary Software, release 8.01. SAS Institute Inc., Cary, NC, USA
Schneller JJ, Holderegger R (1996) Genetic variation in small, isolated fern populations. J Veg Sci 7: 113–120
Sehgal D, Raina SN (2005) Genotyping safflower (Carthamus tinctorius) cultivars by DNA fingerprints. Euphytica 146: 67–76
Shannon CE, Weaver W (1949) The Mathematical Theory of Communication. University of Illinois Press, Urbana, IL, USA
Slatkin M (1987) Gene flow and geographic structure of natural populations. Science 236: 787–792
Spiegelhalter DJ, Best NG, Carlin BP, van der Linde A (2002) Bayesian measures of model complexity and fit. J R Stat Soc [Ser. B] 64: 483–489
Swofford DL (1998) PAUP. Phylogenetic Analysis Using Parsimony and Other Methods, ver. 4.02. Sinauer Associates Inc., Sunderland, MA, USA
Tang S, Bin X, Li W, Zhong Y (2006) Genetic diversity and population structure of yellow camellia (Camellia nitidissima) in China as revealed by RAPD and AFLP markers. Biochem Genet 44: 449–461
Taylor ML, Osborn JM (2006) Pollen ontogeny inBrasenia (Cabombaceae, Nymphaeales). Amer J Bot 93: 344–356
Tero N, Aspi J, Siikamaki R Jakalaniemi A, Tuomi J (2003) Genetic structure and gene flow in a metapopulation of an endangered plant species,Silene tatarica. Mol Ecol 12: 2073–2085
Uehara K, Tanaka N, Momohara A, Zhou Z-K (2006) Genetic diversity of an endangered aquatic plant,Potamogeton lucens subspeciessinicus. Aquat Bot 85: 350–354
Vos R, Hogers R, Bleeker M, van de Lee T, Hornes M, Fritgers A, Pot J, Pelemon J, Kuiper M, Zabeau M (1995) AFLP: A new technique for DNA fingerprinting. Nucl Acids Res 23: 4407–4414
Waycott M, Barnes PAG (2001) AFLP diversity within and between populations of the Caribbean seagrassThalassia testudinum (Hydrocharitaceae). Mar Biol 139: 1021–1028
Wright S (1951) The genetical structure of populations. Ann Eugen 15: 323–354
Xue J, Zhuo L, Zhou S (2006) Genetic diversity and geographic pattern of wild lotus (Nelumbo nucifera) in Heilongjiang Province. Chin Sci Bull 51: 421–432
Yeh F, Yang RC, Boyle T (1997) POPGENE. A User-friendly Shareware for Population Genetic Analysis, ver. 1.31. Molecular and Biotechnology Center, University of Alberta, Edmonton, Alberta, Canada
Zawko G, Krauss SL, Dixon KW, Sivasithamparam K (2001) Conservation genetics of the rare and endangeredLeucopogon obtectus (Ericaceae). Mol Ecol 10: 2389–2396
Zhang D, Chen S, Chen S, Zhang D, Gao Q (2007) Patterns of genetic variation inSwertia przewalskii, an endangered species of the Qinghai-Tibet Plateau. Biochem Genet 45: 33–50
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Kim, C., Na, H.R. & Choi, HK. Conservation genetics of endangeredBrasenia schreberi based on RAPD and AFLP markers. J. Plant Biol. 51, 260–268 (2008). https://doi.org/10.1007/BF03036125
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DOI: https://doi.org/10.1007/BF03036125