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The catecholaminergic RCSN-3 cell line: A model to study dopamine metabolism

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Abstract

RCSN-3 cells are a cloned cell line derived from the substantia nigra of an adult rat. The cell line grows in monolayer and does not require differentiation to express catecholaminergic traits, such as (i) tyrosine hydroxylase; (ii) dopamine release; (iii) dopamine transport; (iv) norepinephrine transport; (v) monoamine oxidase (MAO)-A expression, but not MAO-B; (vi) formation of neuromelanin; (vii) vesicular monoamine transporter-2 (VMAT-2) expression. In addition, this cell line expresses serotonin transporters, divalent metal transporter, DMT1, dopamine receptor 1 mRNA under proliferating conditions, and dopamine receptor 5 mRNA after incubation with dopamine or dicoumarol. Expression of dopamine receptors D2, D3 and D4 mRNA were not detected in proliferating cells or when the cells were treated with dopamine, CuSO4, dicoumarol or dopamine-copper complex. Angiotensin II receptor mRNA was also found to be expressed, but it underwent down regulation in the presence of aminochrome. Total quinone reductase activity corresponded 94% to DT-diaphorase. The cells also express antioxidant enzymes such as superoxide dismutase, catalase and glutathione peroxidase. This cell line is a suitablein vitro model for studies of dopamine metabolism, since under proliferating conditions the cells express all the pertinent markers.

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Authors and Affiliations

  1. Faculty of Medicine, Molecular and Clinical Pharmacology, ICBM, Santiago-7, 70000, Casilla, Chile

    Irmgard Paris, Jorge Lozano, Sergio Cardenas, Carolina Perez-Pastene, Katherine Saud, Patricio Fuentes, Pablo Caviedes, Alexies Dagnino-Ubiabre, RaÚl Caviedes & Juan Segura-Aguilar

  2. INSERM U679, Neurology and Experimental Therapeutics, Hôpital de la Salpêtrière, Université Pierre et MarieCurie-Paris, France

    Rita Raisman-Vozari

  3. Departments of Pharmacology, East Tennessee State University, Johnson City, TN, USA

    John P. Kostrzewa & Richard M. Kostrzewa

  4. Internal Medicine, Quillen College of Medicine, East Tennessee State University, Johnson City, TN, USA

    David Chi

  5. NBCM, CNRS, Gif sur Yvette, France

    Takeshi Shimahara

Authors
  1. Irmgard Paris
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  2. Jorge Lozano
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  3. Sergio Cardenas
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  4. Carolina Perez-Pastene
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  5. Katherine Saud
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  6. Patricio Fuentes
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  7. Pablo Caviedes
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  9. Rita Raisman-Vozari
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  10. Takeshi Shimahara
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  12. David Chi
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  13. Richard M. Kostrzewa
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  14. RaÚl Caviedes
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  15. Juan Segura-Aguilar
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Corresponding author

Correspondence to Juan Segura-Aguilar.

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†Please address correspondence regarding use of the RCSN-3 line to Pablo Caviedes (pcaviede@med.uchile.cl)

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Paris, I., Lozano, J., Cardenas, S. et al. The catecholaminergic RCSN-3 cell line: A model to study dopamine metabolism. neurotox res 13, 221–230 (2008). https://doi.org/10.1007/BF03033505

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  • DOI: https://doi.org/10.1007/BF03033505

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