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High production of SPARC/osteonectin/BM-40 in mouse metastatic B16 melanoma cell lines

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Pathology Oncology Research

Abstract

Production of SPARC/osteonectin/BM-40 was determined in mouse B16 melanoma clones BL6 and F10 (high metastatic) and Fl (low metastatic). SPARC was produced greater amount in BL6 and F10 than in Fl cells, showing a good agreement with their metastatic potentials. Moreover, SPARC production was not influenced by culture pH, even in the acidic conditions (≈pH 5.9). Although tumor tissues show often low pH due to excessive amount of acidic metabolites such as lactate, most studies have been done in neutral pH. High SPARC production in the acidic medium, therefore, is thought to be an important potential for tumor invasive behaviour.

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Authors and Affiliations

  1. Laboratory of Tumor and Developmental Biology, University of Liege, Faculty of Medicine, Liege, Belgium

    Yasumasa Kato, Francis Frankenne, Agnés Noël & Jean -Michel Foidart

  2. Department of Urology, Yokohama City University School of Medicine, Yokohama, Japan

    Naoki Sakai

  3. Department of Pathology, Yokohama City University School of Medicine, Yokohama, Japan

    Yoji Nagashima

  4. Department of Biochemistry, Kanagawa Dental College, 82 Inaoka-cho, 238-8580, Yokosuka, Japan

    Yasumasa Kato & Shinri Koshika

  5. Division of Cell Biology, Kihara Institute for Biological Research, Yokohama City University, Yokohama, Japan

    Kaoru Miyazaki

Authors
  1. Yasumasa Kato
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  2. Francis Frankenne
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  3. Agnés Noël
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  4. Naoki Sakai
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  5. Yoji Nagashima
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  6. Shinri Koshika
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  7. Kaoru Miyazaki
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  8. Jean -Michel Foidart
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Correspondence to Yasumasa Kato.

Additional information

This work was supported by Public Trust Haraguchi Memorial Cancer Research Found, Japan.

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Kato, Y., Frankenne, F., Noël, A. et al. High production of SPARC/osteonectin/BM-40 in mouse metastatic B16 melanoma cell lines. Pathol. Oncol. Res. 6, 24–26 (2000). https://doi.org/10.1007/BF03032654

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  • DOI: https://doi.org/10.1007/BF03032654

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