Abstract
Two cDNA clones,MdCOL1 andMdCOL2, encoding CONSTANS (CO)-like proteins were isolated from an apple (Malus domestica cv. Fuji) fruit cDNA library. Both proteins contain two zinc finger motifs at the amino terminal end and a putative nuclear localization domain at the carboxyl terminal end. Genomic DNA blot analysis suggests that theCO-like genes are members of a small multigene family. RNA blot and RT-PCR analyses revealed that these genes are expressed in every organ that was examined. However, the expression levels were higher in floral buds and fruits at their early developmental stages compared to late reproductive stages or vegetative organs. Such expression patterns are quite different from those of theCO-like genes fromArabidopsis, which show strong organ specificity in either roots, cauline leaves, or flowers. These results indicate that the appleCO-like genes are significantly different from theArabidopsis genes and that they appear to play important roles in reproductive organ development.
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Literature Cited
Blazquez MA (1997) Illuminating I lowers:CONSTANS inducesLEAfY expression. Bioessays19: 277–279
Church G, Gilbert W (1984) Genomic sequencing. Proc Nat! Acad Sci USA81: 1991–1995
Coupland G (1997) Regulation of flowering by photope-riod inArabidopsis. Plant Cell and Environment20:785–789
Daniel-Vedele F, Caboche M (1993) A tobacco cDNA clone encoding a GATA-1 zinc linger protein homologous to regulators of nitrogen metabolism in fungus. Mol Gen Genet240: 365–373
De Paolis A, Sabatini S, De Pascalis L, Costantino P, Capone I (1996) A rolB regulatory factor belongs to a new class of single zinc linger plant proteins. Plant J10: 215–223
Evans RM, Hollenberg SM (1988) Zinc fingers: gilt by association. Cell52: 1–3
Gillaspy G, Ben-David H, Gruissem W (1993) Fruits: A developmental perspective. Plant Cell5: 1439–1451
Harriman R, Tieman D, Handa A (1991) Molec ular cloning of tomato pectin methylesterase gene and its expression in rutgers, ripening inhibitor, nonripening, and never ripe tomato fruits. Plant Physiol97: 80–87
Hecht V, Stiefel V, Delseny M, Gallois P (1997) A newArabidopsis nucleic-ac id-binding protein gene is highly expressed in dividing cells during development. Plant Mol Biol34: 119–124
Ledger S, Dare AP, Putterill JJ (1996) COL2 is a homo-logue of the Arabidopsis flowering-time geneCON STANTS. Plant Physiol112: 862
Lippuner V, Cyert MS, Gasser CS (1996) Two classes of plant cDNA clones differentially complement yeast calcineurin mutants and increase salt tolerance of wild-type yeast. J Biol Chem271: 12859–12866
Meissner R, Michael AJ (1997) Isolation and characterization of a diverse family ofArabidopsis two-and three-fingered C2H2 zinc finger protein genes and cDNAs. Plant Mol Biol33: 615–624
Mitchell PJ, Tjian R (1989) Transcriptional regulation in mammalian cells by sequence-specific DNA binding proteins. Sc ience245: 371–378
Pavletich NP, Pabo CO (1991) zinc: finger-DNA recognition: crystal structure of a zif268-DNA complex at 2.1 A. Science252: 809–817
Putterill JJ, Ledger SE, Lee K, Robson F, Murphy G, Coupland G (1997) The flowering-time geneCONSTANS and homologueCONSTANS like 1 exist as a tandem repeat on c hromosome 5 ofArabidopsis. Plant Physiol114:396
Putterill JJ, Robson F, Lee K, Simon R, Coupland G (1995) TheCONSTANS gene olArabidopsis promotes flowering and encodes a protein showing similarities to zinc finger transcription factors. Cell80: 847–857
Raikhel N (1992) Nuclear targeting in plants. Plant Physiol100: 1627–1632
Ray J, Knapp J, Grierson D, Bird C, Schuch W (1988) Identification and sequence determination of a cDNA for tomato pectinesterase. Eur J Bicx hem174: 119–124
Rogers S,Bendich A (1988) Extraction of DNA from plant tissues. Plant Molecular Biology Manual A6/1–1 I
Salts Y, Wachs R, Gruissem W, Barg R (1991) Sequence coding lor a novel proline-ric h protein preferentially expressed in young tomato fruit. Plant Mol Biol17: 149–150
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
Sanchez-Garcia I, Rabbitts TH (1994) The LIM domain: a new structural motif found in zinc-finger-like proteins. Trends Genet10: 315–320
Sanger F, Nicklen S, Coulson A (1977) DNA sequencing with chain terminating inhibitors. Proc Natl Acad Sci USA74: 5463–5467
Santino CG, Stanford GL, Conner TW (1997) Developmental and transgenic analysis of two tomato fruit enhanced genes. Plant Mol Biol33: 405–416
Sherf B, Kolattukudy P (1993) Developmentally regulated expression of the wound-and pathogen-responsive tomato anionic peroxidase in green fruit. Plant J3: 829–833
Simon R, Igeno Ml, Coupland G (1996) Activation of floral meristem identity genes inArabidopsis. Nature384: 59–62
Sung S, An G (1997) Molecular cloning and characterization of a MADS-box cDNA clone of the Fuji apple. Plant Cell Physiol38: 484–489
Sung S, An G (1997) Trends of genetic study of forest tree and development of useful traits for apple biotechnology. J Plant Biol40: 164–169
Sung S, Jeong D, Nam J, Kim S, Kim S, An G (1998) Expressed sequenced tags of fruits, peels, and carpels and analysis of mRNA expression levels of the tagged c DIM As of fruits from the Fuji apple. Mol Cells8: 565–577
Tague B, Goodman H (1995) Characterization of a family ofarabidopsis zinc finger protein cDNAs. Plant Mol Biol28: 267–279
Tague BW, Gallant P, Goodman HM (199b) Expression analysis of anArabidopsis C2H2 zinc finger protein gene. Plant Mol Biol32: 785–796
Takatsuji H, Nakamura N, Katsumoto Y (1994) A new family of zinc finger proteins in petunia: structure DNA sequence recognition, and floral organ-specific expression. Plant Cell6: 947–958
Yanagisawa S (1995) A novel DNA-binding domain that may form a single zinc finger motif. Nucleic Acids Res23: 340–3410
Wang C, Vodkin L (1994) Extraction of RNA from tissues containing high levels of procyanidins that bind RNA. Plant Mol Biol12: 132–145
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Jeong, DH., Sung, SK. & An, G. Molecular cloning and characterization of constans-like cDNA clones of the fuji apple. J. Plant Biol. 42, 23–31 (1999). https://doi.org/10.1007/BF03031143
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DOI: https://doi.org/10.1007/BF03031143