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Note: Comparison of three laboratory methods to evaluate the pathogenicity and virulence of tenPseudomonas syringae pv.syringae strains on apple, pear, cherry and peach trees

  • Phytopathology/Mycology
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Abstract

The pathogenicity and virulence of ten GreekPseudomonas syringae pv.syringae strains from different hosts (citrus, pear, apple, peach and cherry) were evaluated using three different laboratory methods, which produced results in good agreement. All ten strains were virulent on apple, pear, cherry and peach trees. The extent of tissue colonized varied considerably among strains and cultivars. On excised shoots and twigs of apple and pear, strains BPI 176, BPI 203, PI 2 and PI 14 were the most virulent and strains BPI 689, BPI 992, BPI 4, BPI 20, PI 18 and PI 19 were the least virulent. On excised shoots and twigs of peach and cherry, strains BPI 176, BPI 203, PI 2, PI 14, PI 18 and PI 19 were the most virulent and strains BPI 4 and BPI 20 were the least virulent. Moderate virulence was evinced by strains BPI 689 and BPI 992. These pathogenicity assays are proposed as rapid and reproducible screening systems to evaluate the susceptibility of apple, pear, cherry and peach cultivars to this bacterial pathogen.

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References

  1. Dalla Serra, M., Fagiuoli, G., Nordera, P., Bernhart, I., Della Volpe, C., DiGiorgio, D.et al. (1999) The interaction of lipodepsipeptide toxins fromPseudomonas syringae pv.syringae with biological and model membranes: A comparison of syringotoxin, syringomycin, and two syringopeptins.Mol. Plant-Microbe Interact. 12: 391–400.

    Article  PubMed  CAS  Google Scholar 

  2. Krzesinska, E.Z. and Azarenko, A.N. (1992) Excised twig assay to evaluate cherry rootstocks for tolerance toPseudomonas syringae pv.syringae.HortScience 27: 153–155.

    Google Scholar 

  3. Little, E.L., Bostock, R.M. and Kirkpatrick, B.C. (1998) Genetic characterizationof Pseudomonas syringae pv.syringae strains from stone fruits in California.Appl. Environ. Microbiol. 64: 3818–3823.

    PubMed  CAS  Google Scholar 

  4. Moragrega, C., Llorente, I., Manceau, C. and Montesinos, E. (2003) Susceptibility of European pear cultivars toPseudomonas syringae pv.syringae using immature fruit and detached leaf assays.Eur. J. Plant Pathol. 109: 319–326.

    Article  CAS  Google Scholar 

  5. Scheck, H.J., Canfield, M.L., Pscheidt, J.W. and Moore, L.W. (1997) Rapid evaluation of pathogenicity inPseudomonas syringae pv.syringae with a lilac tissue culture bioassay and syringomycin DNA probes.Plant Dis. 81: 905–910.

    Article  Google Scholar 

  6. Thomidis, T. (2000) Field susceptibility of four peach rootstocks toPhytophthora citrophthora andP. syringae.Phytopathol. Mediterr. 39: 404–409.

    Google Scholar 

  7. Vicente, J.G., Alves, J.P., Russell, K. and Roberts, S.J. (2004) Identification and discrimination ofPseudomonas syringae isolates from wild cherry in England.Eur. J. Plant Pathol. 110: 337–351.

    Article  CAS  Google Scholar 

  8. Yessad, S., Manceau, C. and Luisetti, J. (1992) A detached leaf assay to evaluate virulence and pathogenicity of strains ofPseudomonas syringae pv.syringae on pear.Plant Dis. 76: 370–373.

    Google Scholar 

  9. Yessad-Carreau, S., Manceau, C. and Luisetti, J. (1994) Occurrence of specific reactions induced byPseudomonas syringae pv.syringae on bean pods, lilac and pear plants.Plant Pathol. 43: 528–536.

    Article  Google Scholar 

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Thomidis, T., Tsipouridis, C., Exadaktylou, E. et al. Note: Comparison of three laboratory methods to evaluate the pathogenicity and virulence of tenPseudomonas syringae pv.syringae strains on apple, pear, cherry and peach trees. Phytoparasitica 33, 137–140 (2005). https://doi.org/10.1007/BF03029971

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  • DOI: https://doi.org/10.1007/BF03029971

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