Abstract
Human peritoneal macrophages were collected from dialysis bags of renal patients on Continuous Ambulatory Peritoneal Dialysis (CAPD), during an inflammation-free period. The macrophage suspension was cultured in presence of bacterial lipopolysaccharide (LPS) and phorbol myristate acetate (TPA). The cultured macrophages were tested for therapeutic effectiveness against a human tumor-cell fine, RC43, implanted subcutaneously in NMRI nude mice. The macrophages were injected around the tumor starting from the 14th day after inoculation, when the tumor growth was already detectable (mean tumor size 7 mm). Three injections of macrophages on days 14, 18 and 21 induced hemorrhagic patches at the tumor site and almost complete regression of the tumor. One injection of macrophages cultured either in presence of LPS + TPA or of LPS + TPA + PGE2 resulted in marked slow-down of the tumor growth. Injection of either TNF-alpha (4000 U/mouse) or of PGE2 (150 ng/mouse) given at the site of the palpable small tumor had no effect. Macrophages cultured in medium or in medium supplied with either TPA, LPS or TPA + LPS, were not effective in nude mice bearing large (16 to 19 mm) tumors. The results obtained suggest that activated human macrophages might be therapeutically effective at certain stages of human cancer.
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References
Maddox Y, Foegh M, Zeligs B, Zmudka M, Bellanti J, Ramwell P A: A routine source of human peritoneal macrophages.Scand. J. Immunol.,19, 23–29 (1984).
Ben-Efraim S, Tak C, Fieren M J W A, Bemd van den G I C M Bonta I L: Inflammation amplifies the anttumor cytostasis by human peritoneal macrophages.Med. Oncol. Tumor Pharmacother.,8, 87–94 (1991).
Bonta I L, Ben-Efraim S, Mozes T, Fieren M J W A: Tumour Necrosis Factor in inflammation: relation to other mediators and to macrophage antitumour defence.Pharmacol. Res.,24, 115–130 (1991).
Ben-Efraim S, Tak C, Fieren M J W A, Romijn J C, Beckmann I, Bonta I L: Activity of human peritoneal macrophages against a human tumor: role of Tumor Necrosis Factor, PGEz and nitrite.In vitro studies.Immunology Lett., (in press).
Grossi F S, Zhao X, Romijn J C, Ten-Kate F J W, Schroeder F C S: Metastatic potential of human renal carcinoma; an experimental model using subrenal capsule implantation in nude mice.Urol. Res.,20, 303–306 (1992).
Andreesen R, Brugger W, Gadd S, Lohr G W, Atkins R C: Activation of human monocyte-derived macrophages cultured on teflon: response to interferon during terminal maturationin vitro.Immunobiology,177, 186–198 (1988).
Dumont S, Hartmann D, Poindron P, Oberling F, Faradji A, Bartholenys J: Control of the anttumor activity of human macrophages produced in large amounts in view of adoptive transfer.Eur. J. Clin. Oncol.,21, 1691–1698 (1988).
Fieren M J W A, Bemd van den G J C M, Bonta I L: Endotoxin stimulated peritoneal macrophages obtained from Continuous Ambulatory Peritoneal Dialysis patients show an increased to release interleukin-1in vitro during infectious peritonitis.Eur. J. Clin. Invest.,20, 453–457 (1990).
Cochrane F R, Arietta M B: Regulation of interleukin-1 and tumor necrosis secretion by the human monocyte leukemia cell line, THP-1.Agents and Actions,27, 271–273 (1989).
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Ben-Efraim, S., Tak, C., Romijn, J.C. et al. Therapeutical effect of activated human macrophages on a human tumor line growing in nude mice. Med Oncol 11, 7–12 (1994). https://doi.org/10.1007/BF02990085
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DOI: https://doi.org/10.1007/BF02990085