Abstract
Nine known diarylheptanoids (1–9) isolated from the barks ofAlnus japonica were evaluated for their inhibitory activities on nitric oxide (NO) and prostagrandin E2 (COX-2) production in interferon-γ (INF-γ) and lipopolysaccharide (LPS)-activated RAW 264.7 cellsin vitro. The NO and COX-2 levels were moderately reduced by the addition of compounds (1–9). Among these compounds, compounds6 and8 inhibited NO production in a dose dependent manner with an IC50of 16.7 and 27.2 μg/mL, respectively (positive control, L-NMMA; 22.8 μg/mL), and compounds6,7,8, and9 reduced the COX-2 level in a dose dependent manner with an IC50 of 20.7, 25.7, 25.0, and 27.3 μg/mL, respectively (positive control, indomethacin; 26.2 μg/mL). An analysis of the structural activity relationship among these diarylheptanoids suggests that the presence of a keto-enol group in the heptane moiety or a caffeoyl group in the aromatic ring were important for the efficacy on the inhibitory activities of NO and COX-2 production.
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Kim, HJ., Yeom, SH., Kim, MK. et al. Nitric oxide and prostaglandin E2 synthesis inhibitory activities of diarylheptanoids from the barks ofAlnus japonica steudel. Arch Pharm Res 28, 177–179 (2005). https://doi.org/10.1007/BF02977711
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DOI: https://doi.org/10.1007/BF02977711