Abstract
Brazilin was examined for its effects on the induction of immunological tolerance. Brazilin was administered to C57BL/6 female mice for 2 consecutive days before the immunization with high dose SRBC (109 cells) which can produce immunological tolerance. Delayed type hypersensitivity, IgM plaque forming cells, ConA induced IL-2 production and mitogen- or antigen-induced proliferation of lymphocytes were measured as evaluation parameters. Administration of brazilin prior to immunization could keep the DTH and IL-2 production almost optimaly immunized levels. Brazilin also inhibited the elevation of non-specific suppressor cell activity. ConA induced proliferation of splenocytes in high dose SRBC immunized mice was significantly decreased by pretreatment of brazilin. And this might be one of the reason for augmentation of DTH by brazilin. However, IgM plaque forming cells were not affected by the treatment of brazilin. These results indicate that brazilin prevents the induction of immunological tolerance caused by high dose SRBC by suppressing the elevation of suppressor cell activity and by inhibiting the decrease in IL-2 production in C57BL/6 female mice.
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References
Asherson, G. L. and Colizzi, V., The role of IL-2 as one of the determinants of the balance between immunity and unresponsiveness.Folia Biol., 31, 387–95 (1985).
Askenase, P. W., Hayden, B. J. and Gershon, R. K., Augmentation of DTH by doses of CY which do not affect antibody responses.J. E. M., 141, 697–702 (1975).
Bach, B. A., Sherman, L., Benacerrf, B. and Breene, M. I., Mechanisms of regulation of cell mediated immunity. II. Induction and suppression of delayed type hypersensitivity to azobenearsonate, coupled syngeneic spleen cells.J. Immunol., 121, 1460–1467 (1978).
Baker, D., Karker, K., Antoniou, A. V., Turk, J. L., Tan, B. T. S. and Sheper, R. J., Changes in lymphocyte subsets after treatment with cyclophosphamide and during the development of contact sensitivity in the guinea pig.Int. J. Immunopharmacol., 9, 175–83 (1987).
Choi, S. Y., Yang, K. M., Jeon, S. D., Kim, J. H., Khil, L. Y., Chang, T. S. and Moon, C. K., Brazilin modulates immune function mainly by augmenting T cell activity in halothane administered micePlanta Med., 63, 400–404 (1997a).
Choi, S. Y. and Moon, C. K., Effects of brazilin on the altered immune functions in the early phase of halothane intoxication of C57BL/6 mice.Planta Med., 63, 405–408 (1997b).
Cunningham, A. J. and Szenberg, A., Further improvement in the plaque techniques for detecting single antibody forming cells.Immunol., 14, 599–600 (1968).
Gershon, R. K., A disquisition on suppressor T cells.Transplantation Rev., 26, 170 (1975).
Hoon, D. S. B., Wen, D. R., Stene, M., Gupta, R. K. and Cochran, A. J., Inhibition of lymphocyte mobility by IL-2.Clin. Exp. Immunol., 66, 566–73 (1986).
Ikezawa, Z., Sato, M. and Aoki, I., A role of L3T4 antigen in the ConA response of regenerating splenic L3T4 T cells after CY treatment.Immunol., 60, 375–82 (1987).
Jaffee, B. D., Kerr, J. S., Jones, E. A., Giannaras, J. V., McGowan, M. and Ackerman, N. R., The effect of immunomodulating drugs on adjuvant induced arthritis in Lewis rats.Agents and Actions., 27, 344–6 (1989).
Kramer, M. and Koszinowski, U., T cell specific suppresor factor with regulatory influence on IL-2 production and function.J. Immunol., 128, 784–90 (1982).
Laglange, P. H., Mackaness G. B. and Miller T. E., Potentiation of T cell mediated immunity by selective suppression of antibody formation with CY.J. Exp. Med., 139, 1529–39 (1974).
Malkovsky, M., Asherson, G. L., Stockinger, B. and Watkins M. C., Nonspecific inhibitor released by T acceptor cells reduces the production of IL-2.Nature, 300, 652–5 (1982).
Muettler, D. L., Jenkins, M. K. and Schwartz, R. H., Clonal expansion versus functional clonal inactivation; A costimulatory signalling pathway determines the outcome of T cell antigenic receptor occupancy.Ann. Rev. Immunol., 7, 445–80 (1989).
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Mok, MS., Jeon, SD., Yang, KM. et al. Effects of brazilin on induction of immunological tolerance by sheep red blood cells in C57BL/6 female mice. Arch. Pharm. Res. 21, 769–773 (1998). https://doi.org/10.1007/BF02976774
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DOI: https://doi.org/10.1007/BF02976774