We studied the expression of activation markers CD25 and CD69 by blood lymphocytes in white mice vaccinated with Brucella abortus 19 BA in antigen-specific tests in vitro. During incubation of blood lymphocytes with brucellosis polysaccharide-protein antigen, a statistically significant increase in the expression of CD25 by B cells and CD69 by T cells was observed; brucellin increased the expression of CD25 by B and T cells. Comparative analysis of the action of antigen preparations B. abortus showed that only brucellin has antigen-specific activity against CD19+CD25+ cells. The used method can be considered as a promising test for evaluation of the effectiveness of brucellosis immunoprophylaxis, which substantiates the need for further research.
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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 169, No. 4, pp. 468-471, April, 2020
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Voitkova, V.V., Korytov, K.M., Dubrovina, V.I. et al. Antigen-Specific Activation of Blood Lymphocytes in Mice Immunized with Brucella abortus 19 BA. Bull Exp Biol Med 169, 483–485 (2020). https://doi.org/10.1007/s10517-020-04914-6
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DOI: https://doi.org/10.1007/s10517-020-04914-6