Skip to main content
SpringerLink
Log in

A new thermostable endoglucanase,Acidothermus cellulolyticus E1

Synergism withTrichoderma reesei CBH I and comparison toThermomonospora fusca E5

  • Session 2 Applied Biological Research
  • Published:
Applied Biochemistry and Biotechnology Aims and scope Submit manuscript

Abstract

A new thermostable endoglucanase,Acidothermus cellulolyticus E1, and another bacterial endoglucanase, E5 fromThermomonospora fusca, each exhibit striking synergism with a fungal cellobiohydrolase (Trichoderma reesei CBH I) in the saccharification of microcrystalline cellulose. In neither case did the ratio of endoglucanase to exoglucanase that demonstrated maximum synergism coincide exactly with the ratio that actually released the maximum quantity of soluble sugar for a given total cellulase loading. The difference between the two ratios, after significant hydrolysis of the substrate, was considerably larger in the case ofA. cellulolyticus E1. For both endoglucanase pairings with CBH I, the offset between the ratio for maximum synergism and the ratio for maximal soluble sugar production was found to be a function of digestion time.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Tucker, M. P., Mohagheghi, A., Grohmann, K., and Himmel, M. E. (1989),Bio/Technology 7, 817–820.

    Article  CAS  Google Scholar 

  2. United States Patent 826,089 (CIP); WO93/15186, allowed March 1993; entitled “Thermostable E1 Endoglucanase fromAcidothermus cellulolyticus≓ M. E. Himmel, W. S. Adney, K. Grohmann, and M. P. Tucker.

  3. Wilson, D. (1988),Methods Enzymol. 160, 314–322.

    Article  CAS  Google Scholar 

  4. Calza, R. E., Irwin, D. C., and Wilson, D. B. (1985),Biochemistry 24, 7797–7804.

    Article  CAS  Google Scholar 

  5. Ho. Y.-J. and Wilson, D. B. (1988),Gene 71, 331–337.

    Article  Google Scholar 

  6. Wilson, D. B. (1992),CRC Critical Reviews in Biotechnology 12, 45–63.

    Article  CAS  Google Scholar 

  7. Grohmann, K., Wyman, C. E., and Himmel, M.E. (1992), inEmerging Technologies for Materials and Chemicals from Biomass, Rowell, R. M., Schultz, T. P., and Narayan, R., eds., American Chemical Society (ACS) Symposium Series 476, pp. 354–392.

  8. Henrissat, B., Driguez, H., Viet, C., and Schulein, M. (1985),Bio/Technology 3, 722–726.

    Article  CAS  Google Scholar 

  9. Beldman, G., Voragen, A. G. J., Rombouts, F. M., and Pilnik, W. (1988),Biotechnol. Bioeng. 31, 173–178.

    Article  CAS  Google Scholar 

  10. Woodward, J., Lima, M., and Lee, N. E. (1988),Biochem. J. 255, 895–899.

    CAS  Google Scholar 

  11. Woodward, J., Hayes, M. K., and Lee, N. E. (1988),Bio/Technology 6, 301–304.

    Article  CAS  Google Scholar 

  12. Wood, T. M., McCrae, S. I., and Bhat, K. M. (1989),Biochem. J. 260, 37–43.

    CAS  Google Scholar 

  13. Nidetzky, B., Hayn, M., Macarron, R., and Steiner, W. (1993),Biotech. Lett. 15, 71–76.

    Article  CAS  Google Scholar 

  14. Baker, J. O. and Himmel, M. E. (1991), inEnzymes in Biomass Conversion, Leatham, G. F. and Himmel, M. E., eds., ACS Symposium Series460, American Chemical Society, Washington, DC, pp. 313–330.

    Google Scholar 

  15. Shoemaker, S., Watt, K., Tsitowsky, G., and Cox, R. (1983),Bio/Technology 1, 687–690.

    Article  CAS  Google Scholar 

  16. Walker, L. P., Wilson, D. B., Irwin, D. C, McQuire, C., and Price, M. (1992),Biotechnol. Bioeng. 40, 1019–1026.

    Article  CAS  Google Scholar 

  17. Doner, L. W. and Irwin, P. L. (1992),Anal. Biochem. 202, 50–53.

    Article  CAS  Google Scholar 

  18. Baker, J. O. and Himmel, M. E. (1993), inBiocatalyst Design for Stability and Specificity, Himmel, M. E. and Georgiou, G., eds., ACS Series516, American Chemical Society, Washington, DC, pp. 83–101.

    Google Scholar 

  19. Baker, J. O., Tatsumoto, K., Grohmann, K., Woodward, J., Weichert, J. M., Shoemaker, S., and Himmel, M. E. (1992),Appl. Biochem. Biotech. 34/35, 217–231.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Alternative Fuels Division, National Renewable Energy Laboratory, 1617 Cole Blvd., 80401, Golden, CO

    John O. Baker, William S. Adney, Rafael A. Nleves, Steven R. Thomas & Michael E. Himmel

  2. Section of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, NY

    David B. Wilson

Authors
  1. John O. Baker
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. William S. Adney
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Rafael A. Nleves
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Steven R. Thomas
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. David B. Wilson
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Michael E. Himmel
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Baker, J.O., Adney, W.S., Nleves, R.A. et al. A new thermostable endoglucanase,Acidothermus cellulolyticus E1. Appl Biochem Biotechnol 45, 245–256 (1994). https://doi.org/10.1007/BF02941803

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02941803

Index Entries

Search

Navigation