Abstract
A novel two-temperature dilute-acid prehydrolysis of hybrid poplar xylan that exploits the xylan biphasic kinetics at moderate temperatures is described. A lower temperature (140‡C) is applied to hydrolyze the easily hydrolyzable xylan, and a higher temperature (170‡C) is subsequently applied to hydrolyze the remaining xylan. Using a bench-scale percolation reactor, yields of soluble xylose expressed in monomeric xylose equivalents as high as 92% of theoretical have been achieved with only 2% of the xylan being degraded to furfural. The lignocellulosic substrate produced from the pretreatment is readily converted to ethanol at a yield of 94% of theoretical via a simultaneous saccharification and fermentation process in 48 h. In terms of both yield of xylose equivalents and ethanol production level and rate, these improvements are far superior to those previously reported using a single-temperature dilute-acid pretreatment.
Similar content being viewed by others
References
Fan, L. T., Lee, Y. H., and Gharpuray, M. M. (1982),Adv. Biochem. Eng. 23, 157–187.
Grohmann, K., Wyman, C. E., and Himmel, M. E. (1992), inEmerging Technologies for Materials and Chemicals from Biomass, Rowell, R. M., Schultz, T. P., and Narayan, R., eds., ACS Symp. Ser. #476, pp. 354–392.
McMillan, J. (1992),Processes for Pretreating Lignocellulosic Biomass: A Review, NREL/TP-421-4978, National Renewable Energy Laboratory, Golden, CO, pp. 1–44.
Grohmann, K., Torget, R., and Himmel, M. (1985),Biotech. Bioeng. Symp. 15, 59–80.
Grohmann, K., Torget, R., and Himmel, M. (1986),Biotech. Bioeng. Symp. 17, 135–151.
Torget, R., Himmel, M., Wright, J., and Grohmann, K. (1988),Appl. Biochem. Biotech. 17, 89–104.
Torget, R., Werdene, P., Himmel, M., and Grohmann, K. (1990),Appl. Biochem. Biotech. 24, 115–126.
Torget, R., Walter, P., Himmel, M., and Grohmann, K. (1991),Appl. Biochem. Biotech. 28/29, 75–86.
Torget, R., Himmel, M., and Grohmann, K. (1992),Appl. Biochem. Biotech. 34/35, 115–123.
Grohmann, K., Himmel, M., Rivard, C., Tucker, M., Baker, J., Torget, R., and Graboski, M. (1984),Biotech. Bioeng. Symp. 14, 137–157.
Torget, R. (1985), M. S. Thesis, Colorado School of Mines, Golden, CO.
Grethlein, H. E. (1985),Bio. Technology 3, 155–160.
Springer, E. L., Harris, J. F., and Neill, W. K. (1963),TAPPI 46, 551–555.
Springer, E. L. and Zoch, L. L. (1968),TAPPI 51, 214–218.
Kobayashi, T. and Sakai, Y. (1956),Bull Agr. Chem. Soc. Japan 20, 1–7.
Maloney, M. T., Chapman, T. W., and Baker, A. J. (1985),Biotech. Bioeng. 27, 355–361.
Conner, A. H. (1984),Wood Fiber Sci. 16, 268–277.
Carrasco, F. and Roy, C. (1992),Wood Sci. Technol. 26, 189–208.
Schell, D. J., Torget, R., Power, A., Walter, P. J., Grohmann, K., and Hinman, N. D. (1991),Appl. Biochem. Biotech. 28/29, 87–97.
Grohmann, K. and Torget, R. W. (1992), U.S Patent #5,125,977.
Torget, R., Hsu, T., Kim, B. J., and Lee, Y. Y. (1992), AIChE 1992 Annual Meeting, Miami, FL.
Ghose, T. K. (1987),Pure Appl. Chem. 59, 257–268.
Spindler, D. D., Wyman, C. E., Grohmann, K., and Philippidis, G. P. (1992),Biotech. Lett. 14, 403–407.
Official Test Methods (1983),TAPPI, Atlanta, GA.
Moore, W. E. and Johnson, D. B. (1967),Procedures for the Chemical Analysis of Wood and Wood Products, USDA Forest Products Laboratory, Madison, WI.
Technical Association of the Pulp and Paper Industry Standard Method T 250,TAPPI, “Acid-Soluble Lignin in Wood and Pulp.≓
Spindler, D. D., Wyman, C. E., and Grohmann, K. (1991),Appl. Biochem. Biotech. 28/29, 773–786.
Smith, J. M. (1981),Chemical Engineering Kinetics, 3rd ed. McGraw-Hill Book Company, New York.
Abramowitz, M. and Stegun, I. A., eds. (1972),Handbook of Mathematical Functions, 10th printing, National Bureau of Standards, Washington D.C.
Kim, B. J., Lee, Y. Y., and Torget, R. (1993),Appl. Biochem. Biotech. accepted for publication.
Kim, B. J., Lee, Y. Y., and Torget, R. submitted for publication inAppl. Biochem. Biotech.
Lee, Y. Y. (1993), Personal communication, Auburn University, AL.
Tatsumoto, K., Baker, J. O., Tucker, M. P., Oh, K. K., Mohagheghi, A., Grohmann, K., and Himmel, M. E. (1988),Appl. Biochem. Biotech. 18, 159–174.
Hinman, N. D., Schell, D. J., Riley, C. J., Bergeron, P. W., and Walter, P. J. (1992),Appl. Biochem. Biotech. 34/35, 639–649.
Conner, A. H., Wood, B. F., Hill, C. G., Jr., and Harris, J. F. (1985),J. Wood Chem. Tech. 5(4), 461–489.
Burchhardt, G. and Ingram, L. O. (1992),Appl. and Environ. Microb. 58, 1128–1133.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Torget, R., Teh-An, H. Two-temperature dilute-acid prehydrolysis of hardwood xylan using a percolation process. Appl Biochem Biotechnol 45, 5–22 (1994). https://doi.org/10.1007/BF02941784
Issue Date:
DOI: https://doi.org/10.1007/BF02941784