Abstract
The scale-up of inulinase production in aerated cultures ofCandida kefyr DSM 70106 was studied, taking into account the criterion of maintainingK L a constant. The culture was carried out batchwise, in a 15-L fermentor, withK L a varying from 25 to 199 h−1. The highest inulinase production was attained with an initialK L a value of 46 h−1. A large scale fermentation (300-L fermentor) was performed using identical culture medium conditions. The responses obtained for the bench and scaled-up experiments showed similar behaviors, and the results were, respectively, 0.60 and 0.58 U·mL−1·h−1 for productivity and 43.0 and 41.5 U·mL−1 for activities.
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Abbreviations
- Yx/s :
-
ΔX/ΔS
- productivity (Pr):
-
ΔP/Δt
- μx(max) :
-
maximum specific growth rate obtained from the slope of ln(x) as a function of time in the cell growth logarithmic phase
- U·g −1cel (max) :
-
specific enzymatic activity
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Pessoa, A., Vitolo, M. & Hustedt, H. Use ofK L a as a criterion for scaling up the inulinase fermentation process. Appl Biochem Biotechnol 57, 699–709 (1996). https://doi.org/10.1007/BF02941752
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DOI: https://doi.org/10.1007/BF02941752