Summary
The β-fructofuranosidase activities of a strain of Clostridium acetobutylicum, selected for its capacity to grow on inulinic substrates, were investigated. When grown on inulin, this strain produced extracellular and intracellular β-fructofuranosidases, both of which hydrolysed inulin (inulinase activity) and sucrose (invertase activity). Inulinase activity was higher than invertase activity in the extracellular preparation, the opposite being observed for the cellular preparation. The effects of pH and temperature, substrate specificity and the kinetic constants for inulin and sucrose were studied on both preparations, as well as induction by inulin and repression by glucose and fructose of inulinase and invertase activities. The overall results were consistent with the existence of a least one inulinase, (EC 3.2.1.7), mainly but not entirely released in the extracellular medium, and an invertase (3.2.1.26) localized within the cell.
Time course hydrolysis experiments of dalhia inulin and Jerusalem artichoke inulofructans by extracellular inulinase showed that this preparation had a remarkably high specificity for hydrolysis of long chain inulofructans.
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References
Bergmeyer HU, Bernt E, Schmidt F, Stork H (1974) d-glucose. In: Bergmeyer HU (ed) Methods of enzymatic analysis. Verlag Chemie Weinheim Acad Press, New-York, London, pp 1196–1201
Bernt E, Bergmeyer HU (1974) d-fructose. In: Bergmeyer HU (ed) Methods of enzymatic analysis. Verlag Chemie, Weinheim, Acad Press, New York, London, pp 1304–1307
Davies R, Stephenson M (1941) Acetone—butyl alcohol fermentation. I. Nutritional and other factors involved in the preparation of active suspension of C. acetobutylicum (Weizman). Biochem J 35: 1320–1331
Efstathiou R, Reysset G, Truffaut N (1986) A study of inulinase activity in the Clostridium acetobutylicum strain ABKn8. Appl Microbiol Biotechnol 25:143–149
Groot Wassink JWD, Fleming SE (1980) Non-specific β-fructofuranosidase (inulase) from Kluyveromyces fragilis: batch and continuous fermentation, simple recovery method and some industrial properties. Enzyme Microb Technol 2:45–53
Heyraud A, Rinaudo M, Taravel FR (1984) Isolation and characterisation of oligosaccharides containing d-fructose from juice of Jeruscalem artichoke. Kinetic constants for acid hydrolysis. Carbohydrate Research 128:311–320
Laishey EJ (1975) Regulation and properties of an invertase from Clostridium pasteurianum. Canadian J Microbiol 21:1711–1718
Lowry O, Rosebrough N, Farr A, Randall R (1951) Protein measurement with the Folin-phenol reagent. J Biol Chem 193:265–275
Marchal R, Blanchet D, Vandecasteele JP (1985) Industrial optimisation of acetone-butanol fermentation: a study of the utilisation of Jerusalem artichokes. Appl Microbiol Biotechnol 23:92–98
Van Eyk J, Bartels TJ (1970) Paraffin oxidation in Pseudomonas aeruginosa. II. Gross fractionation of the enzyme system into soluble and particulate components. J Bacteriol 104:1065–1073
Vandamme EJ, Derycke DG (1983) Microbial inulinases: fermentation process, properties, and applications. Adv Appl Microbiol 29:139–176
Zittan L (1981) Enzymic hydrolysis of inulin, an alternative way to fructose production. Starch 33:373–377
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Looten, P., Blanchet, D. & Vandecasteele, J.P. The β-fructofuranosidase activities of a strain of Clostridium acetobutylicum grown on inulin. Appl Microbiol Biotechnol 25, 419–425 (1987). https://doi.org/10.1007/BF00253311
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DOI: https://doi.org/10.1007/BF00253311