Summary
Optimisation of a non-equilibrium radioimmunoassay for substance P is described. The assay has a sensitivity of 2 pg (1.5 fmole), sufficient for substance P determinations in unexcepted human plasma and other biological material. The specificity of antisera used was tested with closely related peptides, physalaemin and eledoisin. Crossreaction of 1 per cent for eledoisin and 0.1 per cent for physalaemin was found. The assay is easy to perform, has a precision of 5 per cent, and takes only 24 hours.
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O’Connell, R., Skrabanek, P., Cannon, D. et al. High-sensitivity radioimmunoassay for substance P. IJMS 145, 392–398 (1976). https://doi.org/10.1007/BF02938978
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DOI: https://doi.org/10.1007/BF02938978