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The influence of environmental conditions on the production of pigment bySerratia marcescens

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Abstract

Serratia marcescens biovar A2/A6, isolated from an Indonesian freshwater source, was identified based on extensive morphological, biochemical and genetic characterization. Formation of pigment was found to be strongly influenced by environmental conditions. Placket-Burman design was used to analyze the effect of carbon and nitrogen sources. Based on results of physiological and biochemical studies, the optimum conditions for growth and pigment formation were incubation 30°C in a neutral to slightly alkaline medium containing lactic acid and beef extract.

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Abbreviations

E i,j :

The effect of ith on jth-variable (i=1 to 8 & j=1 to 4)

ni,j (+):

The number of experiments at high concentrations of ith-variable which is equal to 6

ni,j (−):

The number of experiments at low concentrations of ith-variable which is equal to 6

Ri,j (+):

Average final concentration of jth at high concentration of ith-variable

Ri,j (−):

Average final concentration of jth at low concentration of ith-variable

SE i,j :

Standard error of ith on jth-variable (i=1 to 8 & j=1 to 4)

t :

t-value

V i,j :

The variance of ith on jth-variable (i=1 to 8 & j=1 to 4)

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Authors and Affiliations

  1. Center for Coastal and Marine Resources Studies, Bogor Agricultural University and Inter University Research Center on Biotechnology, Bandung Insitute of Technology, Indonesia

    Linawati Hardjito

  2. Center of Marine Biotechnology, University of Maryland Biotechnology Institute, Baltimore, Maryland, USA

    Anwar Huq & Rita R. Colwell

  3. Department of Cell and Molecular Biology, University of Maryland, 20742, College Park, Maryland, USA

    Anwar Huq & Rita R. Colwell

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  1. Linawati Hardjito
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  2. Anwar Huq
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  3. Rita R. Colwell
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Correspondence to Linawati Hardjito.

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Hardjito, L., Huq, A. & Colwell, R.R. The influence of environmental conditions on the production of pigment bySerratia marcescens . Biotechnol. Bioprocess Eng. 7, 100–104 (2002). https://doi.org/10.1007/BF02935887

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  • DOI: https://doi.org/10.1007/BF02935887

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