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Comparing the performance of one-column process and four-zone simulated moving bed by computer simulation

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Abstract

A new one-column chromatography process, analogous to a four-zone simulated moving bed (SMB), was presented. The basic principle of the process was identical to that of a four-zone SMB. The process consisted of one chromatographic column and four tanks, instead of the four columns in the four-zone SMB (1-1-1-1), and has been used for the separation of two amino acids, phenylalanine and tryptophan, using an ion exchange resin. The operating parameters for the one-column process and four-zone SMB were obtained from equilibrium theory. Computer simulations were used to compare the performances of the new one column process to that of the general four-zone SMB, using Aspen Chromatography™ v 11.1. The differences between the one-column and SMB processes in terms of the purities and yields of phenylalanine and tryptophan were less than 4 and about 6%, respectively. The lower purities of the one-column process were due to the loss of the developed concentration profiles in the column when the liquid was stored in tanks. The one-column process gave great flexibility, and would be useful for reconstructing an existing conventional chromatography process to one of a SMB.

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Authors and Affiliations

  1. Department of Biological Engineering, ERC for Advanced Bioseparation Technology, Inha University, 402-751, Incheon, Korea

    Young Sik Kim, Chong Ho Lee & Yoon Mo Koo

  2. School of Chemical Engineering, Purdue University, 47907, West Lafayette, IN, USA

    Phillip C. Wankat

Authors
  1. Young Sik Kim
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  2. Chong Ho Lee
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  3. Phillip C. Wankat
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  4. Yoon Mo Koo
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Correspondence to Yoon Mo Koo.

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Kim, Y.S., Lee, C.H., Wankat, P.C. et al. Comparing the performance of one-column process and four-zone simulated moving bed by computer simulation. Biotechnol Bioproc E 9, 362–368 (2004). https://doi.org/10.1007/BF02933058

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  • DOI: https://doi.org/10.1007/BF02933058

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