Abstract
Parameter estimations were made for the reversed-phase adsorption of perillyl alcohol (POH), a potent anti-cancer agent, on octadecylsilyl-silica gel (ODS). The average particle diameter of ODS was about 15 μm, and the particles were packed in the column (3.9 × 300 mm). The mobile phase used was a mixture of acetonitrile and water, in which the acetonitrile ranged between 50 and 70 (v/v %). The first absolute moment and the second central moment were determined from the chromatographic elution curves by moment analysis. Experiments were carried out using POH solutions within the linear adsorption range. The fluid-to-particle mass transfer coefficient was estimated using the Wilson-Geankoplis equation. The axial dispersion coefficient and the intraparticle diffusivity were determined from the slope and intercept of a plot ofH vs 1/u 0, respectively. The contributions of each mass-transfer step were axial dispersion, fluid-toparticle mass transfer, and intraparticle diffusion.
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Row, K.H., Lee, C.H. & Kang, J.H. Parameter estimation of perillyl alcohol in RP-HPLC by moment analysis. Biotechnol. Bioprocess Eng. 7, 16–20 (2002). https://doi.org/10.1007/BF02935874
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DOI: https://doi.org/10.1007/BF02935874