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Effect of radiation on the biosynthesis of L-Tryptophan from 14C-Anthranilic acid in kohlrabi (Brassica oleracea L., var.gongylodes L.)

Studium vlivu záření na biosyntézu L-tryptofanu z14C-kyseliny antranilové v brukvi (Brassica oleracea var. gongylodes L.)

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Biologia Plantarum

Abstract

The metabolism of14C-anthranilic acid (14C-AA) in kohlrabi (Brassica oleracea L. var.gongylodes L.) and the effect of radiation gamma60Co on this metabolism was investigated. In hypocotylar segmnents of seven days old etiolated seedlings14C-AA was metabolised par, tially to its detoxication product14C-β-glucoside of AA. Simultaneously L-tryptophan was also formed, which in these plants is a precursor of indolic glucosinolates glucobrassicin and neoglucobrassicin.

The metabolism of14C-AA was followed for 97 h. Radiation, applied both to seeds and to seven days old plants did not affect the metabolism of14C-AA substantially. The intermediary reaction AA → L-tryptophan in the biosynthesis of L-tryptophan is not a radiosensitive part of the synthesis of this amino acid. A not too high radiation sensitivity (max. 45%) was observed in the metabolic pathway leading from L-tryptophan to glucobrassicin.

Abstract

Byl studován metabolismus14C-kyseliny antranilové (14C-AA) v brukvi (Brassica oleracea var. gongylodes L.) a vliv záření gama60Co na tento metabolismus. V hypokotylárních segmentech ze sedmidenních etiolovaných rostlin byla14C-AA metabolisována na její detoxikační produkt14C- β glukosid AA, současně se z AA vytvářel i L-tryptofan, který je v těchto rostlinách prekursorom indolových glukosinolátů glukobrasicinu a neoglukobrasicinu.

Metabolismus14C-AA byl sledován během 97 h. Radiace, aplikovaná jednak na semena, jednak na sedmidenní rostliny, neovlivnila podstatně metabolismus14C-AA. Intermediární reakfie AA → L-tryptofan v biosyntéze L-tryptofanu není radiosensitivním úsekem syntézy této aminokyseliny. Nevelkou radiační citlivost (max 45%) jevila m^tabolická dráha vedoucí od L-tryptofanu ke glukobrasicinu.

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References

  • Andreae, W. A., Good, N. E.: The formation of indoleacetylaspartic acid in pea seedlings. — Plant Physiol.30: 380–382, 1955.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Cervigni, T., Belli, M. L.: Modifications de l'acide β-indolacetique par les radiations ionisantes. — In: Biological Effects of Ionizing Radiation at the Molecular Level. Pp. 193–202. IAEA, Vienna 1962.

    Google Scholar 

  • Chen, J., Boll, W. G.: Tryptophan synthetase in shoot and root tissue of pea seedlings. — Canad. J. Bot.46 : 1031–1041, 1968.

    Article  CAS  Google Scholar 

  • Delmer, D. P., Mills, S. E.: Tryptophan biosynthesis in cell cultures ofNicotiana tabacum. — Plant Physiol.43: 81–87, 1968.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Good, N. E., Andreae, W. A., van Ysselstein, M. W. H.: Studies on 3-indoleacetic acid metabolism II. Some products of the metabolism of exogenous indoleacetic acid in plant tissues. — Plant Physiol.31: 231–235, 1956.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Gordon, S. A.: The effect of ionizing radiation on plants: Biochemical and physiological aspects. — Quart. Rev. Biol.32: 3–14, 1957.

    Article  CAS  PubMed  Google Scholar 

  • Klämbt, H. D.: Wachstumsinduktion iund Wuchsstoffmetabolismus im Weizenkoleoptilzylinder. Reaktionsprodukte der Indolessigsäure in Abhängigkeit von den Inkubationskonzentrationen. — Planta56: 309–321, 1961a.

    Article  Google Scholar 

  • Klämbt, H. D.: Wachstumsinduktion und Wuchsstoffmetabolismus im Weizenkoleoptilzylinder II. Stoffwechselprodukte der Indol-3-essigsäure und der Benzoesäure. — Planta56: 618–631, 1961b.

    Article  Google Scholar 

  • Kutáček, M., Galston, A. W.: The metabolism of14C-labelled isatin and anthranilate inPisum stem sections. — Plant Physiol.43: 1793–1798, 1968.

    Article  PubMed Central  PubMed  Google Scholar 

  • Kutáček, M., Kefeli, V.I.: The present knowledge of indole compounds in plants of theBrassicaceaea family. — In:Wightman F., Setterfield, G. (ed.): Biochemistry and Physiology of Plant Growth Substances. Pp. 127–152. Runge Press, Ottawa 1968.

    Google Scholar 

  • Kutáček, M., Kefeli, V. I.: Biogenesis of indole compounds from D- and L-tryptophan in segments of etiolated seedlings of cabbage, maize and pea. — Biol. Plant.12: 145–158, 1970.

    Article  Google Scholar 

  • Kutáček, M., Mašev, N., Oplištilová, K., Bulgakov, R.: The influence of gamma-radiation on the biosynthesis of indoles and gibberellins in barley. The action of zinc on the restitution of growth substance level in irradiated plants. — Biol. Plant.8: 152–163, 1966.

    Article  Google Scholar 

  • Kutáček, M., Mašev, N., Oplištilová, K., Bulgakov, R.: The influence of zinc and gammaradiation on the metabolism of auxins and gibberellins in seven-day old barley andBrassica plants. In Russ. — In: Proc. int. Symp. Plant Stimulation. Pp. 1141–1146. BAN, Sofia 1967.

    Google Scholar 

  • Kutáček, M., Rokosová, K., Řetovský, R.: A study of metabolism of exogenous tryptophan and β-indoleacetic acid in extirpated wheat embryos. — Biol. Plant.1: 54–62, 1959.

    Article  Google Scholar 

  • Kutáček, M.,Schraudolf, H.: Metabolismus14C-markierter Anthranilsäure in Hypokotylsegmenten von Senf(Sinapis alba L.). — Proc. 2nd int. Symp.Isothiocyanates, Smolenice 1969. Pp. 163–173, 1971.

  • Moore, J. N., Hough, L. F.: The influence of gamma irradiation and photoperiod on auxin levels and growth of the strawberry plant. — Bull. Torrey bot. Club89: 381–398, 1962.

    Article  CAS  Google Scholar 

  • Polyanovskiï, O. L., Kretovich, B. L.: [Quantitative determination and biosynthesis of tryptophan in plants]. In Russ. — Dokl. Akad. Nauk SSSR112 : 1086–1089, 1957.

    Google Scholar 

  • Skoog, F.: The effect of X-rays on growth substances and plant growth. — Science79: 256, 1934.

    Article  CAS  PubMed  Google Scholar 

  • Skoog, F.: Effect of X-irradiation on auxin and plant growth. — J. cell. comp. Physiol.7:: 227–270, 1935.

    Article  CAS  Google Scholar 

  • Veen, H.: Transport immobilization and localization of naphthylacetie acid-l-14C in Coleus expiants. — Acta bot. neerl.15: 419–433, 1966.

    Article  CAS  Google Scholar 

  • Wightman, F., Chisholm, M. O., Neish, A. C: Biosynthesis of tryptophan and gramine in young barley shoots. — Phytochemistry1: 30–34, 1961.

    Article  CAS  Google Scholar 

  • Zenk, M. H.: l-(indole-3-acetyl)-β-D-glucose, a new compound in the metabolism of indole-3- acetic acid in plants. — Nature191: 493–494, 1961.

    Article  CAS  PubMed  Google Scholar 

  • Zenk, M. H.: Aufnahme und Stoffwechsel von α-Naphthyl-essigsäure durch Erbsenepikotyle. — Planta58: 75–91, 1962.

    Article  CAS  Google Scholar 

  • Zenk, M. H.: Isolation, biosynthesis and function of indoleacetic acid conjugates. — In: Régulateurs Naturels de la Croissance Végétale. — Pp. 241–249. CNRS Nr. 123, Paris 1964.

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  1. Institute of Experimental Botany, Czechoslovak Academy of Sciences, Ke dvoru 15, Praha 6-Vokovice, Czechoslovakia

    J. Eder & M. Kutáček

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  1. J. Eder
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Eder, J., Kutáček, M. Effect of radiation on the biosynthesis of L-Tryptophan from 14C-Anthranilic acid in kohlrabi (Brassica oleracea L., var.gongylodes L.). Biol Plant 14, 390–398 (1972). https://doi.org/10.1007/BF02932979

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  • DOI: https://doi.org/10.1007/BF02932979

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