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Analysis of cerebrospinal fluid cell populations with monoclonal antibodies

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Abstract

Sixty-five samples of cerebrospinal fluid (CSF) were evaluated using an automated cytoflow method with the CD-Sapphire hematology analyzer in order to investigate possible relationships between cell population patterns and diagnostic groups and better understand the biology of neurological disease. A basic panel of CD markers, including CD3/4/8/19/138/HL A-DR, was used to analyze CSF samples from clinical and laboratory confirmed cases of multiple sclerosis, neuroborreliosis, viral and bacterial neuro-infective diseases, malignant infiltrations of meninges and scavenger macrophagic reactions of the central nervous system. The principles of immune response and the contribution of cytological ‘disease-related patterns’ for these nosological entities are described. The distinct patterns of lymphocyte subpopulations in neuroborreliosis appear to be characteristic and could possibly serve as diagnostic indicators. Further verification and research will be necessary to clarify the significance and nature of CD4+ CD8+ positive subset in cerebrospinal fluid.

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Abbreviations

CD:

cluster of differentiation

CNS:

central nervous system

CSF:

cerebrospinal fluid

IND:

inflammatory neurological diseases

FL:

fluorescent channel

FITC:

fluorescein isothiocyanate

Mab:

monoclonal antibody(ies)

MIM:

malignant infiltration of meninges

MS:

multiple sclerosis

NB:

neuroborreliosis

NIND:

non-inflammatory neurological diseases

PE:

phycoerythrin

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Authors and Affiliations

  1. Laboratory of Reference for Cerebrospinal Fluid and Neuroimmunology, Homolka Hospital, 150 30, Prague, Czechia

    P. Adam & O. Sobek

  2. Abbott Laboratories, Wiesbaden-Delkenheim, Germany

    C. S. Scott

Authors
  1. P. Adam
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  2. O. Sobek
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  3. C. S. Scott
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Correspondence to P. Adam.

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Adam, P., Sobek, O. & Scott, C.S. Analysis of cerebrospinal fluid cell populations with monoclonal antibodies. Folia Microbiol 52, 529–534 (2007). https://doi.org/10.1007/BF02932115

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  • DOI: https://doi.org/10.1007/BF02932115

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