Abstract
Streptomyces caelestis DSM 40084produces two osmolytes,viz. 2-O-(α-d-glucopyranosyl)-ζ-glyceric acid (GG) and trehalose. Both compounds were isolated and identified by nuclear magnetic resonance spectroscopy and mass spectrometry. A very sensitive regulation of the cell osmolytes was demonstrated in exponentially growing cultures. The intracellular levels of GG and trehalose increased 2× in response to a step change of medium osmolarity caused by 0.3 % NaCl.1H NMR analysis of the cell extracts did not confirm the presence of additional osmolytes. GG is aS. caelestis metabolite commonly released from the cells; its concentration reached 3 g/L during the cultivation in a yeast extract-(NH4)2SO4-glycerol medium. This is the first report on the occurrence of the ionic osmolyte GG in the genusStreptomyces and on its free excretion to the medium.
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Abbreviations
- 1D-TOCSY:
-
one-dimensional total-correlated spectroscopy
- CD:
-
chemically defined (medium)
- ESI MS:
-
electrospray ionization mass spectrometry
- GG:
-
2-O-(α-d-glucopyranosyl)-ξ-glyceric acid
- HMBC:
-
heteronuclear multiple bond correlation
- MS:
-
mass spectrometry
- NMR:
-
NMR spectroscopy
- YEA:
-
complex (medium)
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This work was supported by theInstitutional Research Concept AV 0Z 5020 0510 and, in part, by theGrant Agency of the Czech Academy of Sciences (research grant IAA 600 660 607).
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Pospíšil, S., Halada, P., Petříček, M. et al. Glucosylglycerate is an osmotic solute and an extracellular metabolite produced byStreptomyces caelestis . Folia Microbiol 52, 451–456 (2007). https://doi.org/10.1007/BF02932103
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DOI: https://doi.org/10.1007/BF02932103