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Comparison of the bioluminescence ofPhotorhabdus species and subspecies type strains

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Abstract

Natural bioluminescence of all recently acceptedPhotorhabdus species and subspecies type strains (bacterial symbionts of entomopathogenic nematodes) was measured using a commercial luminometer; optimum conditions for the measurement were described. Cultures emitted reliably measurable bioluminescence with characteristic level and kinetics for each strain. Bioluminescence of all strains was significantly higher at 37 than at 25 °C at the beginning of the measurement, no effect of bacterial concentration on the intensity of bioluminescence was observed. The technique can provide reliable and quick information for the determination ofPhotorhabdus taxons.

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Abbreviations

BL:

bioluminescence

HBSS:

Hank’s balanced salt solution

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Authors and Affiliations

  1. Department of Comparative Animal Physiology and General Zoology, Faculty of Science Masaryk University, 611 37, Brno, Czechia

    P. Hyršl

  2. Institute of Biophysics, Academy of Sciences of the Czech Republic, 612 65, Brno, Czechia

    M. Čiž & A. Lojek

Authors
  1. P. Hyršl
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  2. M. Čiž
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  3. A. Lojek
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Corresponding author

Correspondence to A. Lojek.

Additional information

This study was elaborated in the frame of research plan Z 5004920 and supported by grant IGA AS CR S5004009.

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Hyršl, P., Čiž, M. & Lojek, A. Comparison of the bioluminescence ofPhotorhabdus species and subspecies type strains. Folia Microbiol 49, 539–542 (2004). https://doi.org/10.1007/BF02931530

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  • DOI: https://doi.org/10.1007/BF02931530

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