Abstract
A time-correlated expression of eukaryotic-like protein Ser/Thr kinase Pkg2 ofStreptomyces granaticolor was investigated by reverse transcriptase-polymerase chain reaction (RT-PCR) and by transcriptional fusion experiments. In a complex medium the activity ofpkg2 promoter was constant during the life cycle. Direct RNA analysis proved the presence of correspondingpkg2 transcript. S1 nuclease protection analysis of the transcription initiation site showed thatpkg2 gene is expressed as a leaderless mRNA. Under phosphate starvation the promoter activity was detectable merely in the early exponential phase. Under these conditions turning off ofpkg2 promoter and cessation ofpkg2 transcript level coincided with the start of granaticin production.
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This work was supported by theGrant Agency of the Czech Republic (grant 204/02/1423 to the last author) and byInstitutional Research Concept no. AV 0Z 5020 0510.
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Nováková, L., Nádvorník, R., Vomastek, T. et al. Effect of phosphate on the expression of protein-Ser/Thr kinase Pkg2 inStreptomyces granaticolor . Folia Microbiol 50, 503–508 (2005). https://doi.org/10.1007/BF02931438
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DOI: https://doi.org/10.1007/BF02931438