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Transformation ofStreptomyces granaticolor with natural and recombinant plasmid vectors

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Abstract

Protoplasts ofStreptomyces granaticolor were found to be transformable by the broad-host-range plasmid pIJ350 but no transformants were detected when the narrow-host-range plasmid pIJ2 or the shuttle vector pPM66 (pIJ350 — pBR322) isolated fromE. coli cells were used. The onset of blue colour granaticin production byS. granaticolor cells was used as a marker to prepare protoplasts with a high transformation capcity. The presence of a restriction system is discussed.

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Authors and Affiliations

  1. Department of Molecular Biology and Genetics, Institute of Microbiology, Czechoslovak Academy of Sciences, 142 20, Prague 4

    M. Petříček, I. Smrčková & P. Tichý

  2. John Innes Institute, Norwich, England

    D. A. Hopwood

Authors
  1. M. Petříček
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  2. I. Smrčková
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  3. P. Tichý
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  4. D. A. Hopwood
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Petříček, M., Smrčková, I., Tichý, P. et al. Transformation ofStreptomyces granaticolor with natural and recombinant plasmid vectors. Folia Microbiol 30, 474–478 (1985). https://doi.org/10.1007/BF02927609

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  • DOI: https://doi.org/10.1007/BF02927609

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