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Agrobacterium tumefaciens-mediated transformation ofMucor circinelloides

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Abstract

TheAgrobacterium tumefaciens-mediated transformation of the zygomycetous fungusMucor circinelloides is described. A method was also developed for the hygromycin B-based selection ofMucor transformants. Transformation with the hygromycin B phosphotransferase gene ofEscherichia coli controlled by the heterologousAspergillus nidulans trpC promoter resulted in hygromycin B-resistant clones. The presence of the hygromycin resistance gene in the genome of the transformants was verified by polymerase chain reaction and Southern hybridization: the latter analyses revealed integrations in the host genome at different sites in different transformants. The stability of transformants remained questionable during the latter analyses.

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Authors and Affiliations

  1. HAS-USZ Microbiological Research Group, Department of Microbiology, University of Szeged, 6701, Szeged, Hungary

    I. Nyilasi & E. Nagy

  2. Department of Microbiology, Faculty of Science, University of Szeged, 6701, Szeged, Hungary

    K. Ács, T. Papp & C. Vágvölgyi

Authors
  1. I. Nyilasi
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  2. K. Ács
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  3. T. Papp
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  4. E. Nagy
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  5. C. Vágvölgyi
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Additional information

This research was supported in part by theHungarian Scientific Research Fund (OTKA) grants T037471, F046658 and D048537.

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Nyilasi, I., Ács, K., Papp, T. et al. Agrobacterium tumefaciens-mediated transformation ofMucor circinelloides . Folia Microbiol 50, 415–420 (2005). https://doi.org/10.1007/BF02931423

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