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Effect of protein kinase inhibitors on protein phosphorylation and germination of aerial spores fromStreptomyces coelicolor

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Abstract

In vitro phosphorylation reaction using extracts prepared from cells in the exponential phase of growth and aerial spores ofStreptomyces coelicolor displayed the presence of multiply phosphorylated proteins. Effect of protein kinase inhibitors (PKIs) (geldanamycin, wortmannin, apigenin, genistein, roscovitine, methyl 2,5-dihydroxycinnamate, rapamycin, staurosporine) was determined on protein phosphorylation and on germination of spores. Thein vitro experiments showed differences in phosphoprotein pattern due to the presence of PKIs. Cultivation of aerial spores with PKIs led to a significant delay in germ tube emergence and filament formation. However, none of the tested PKIs completely blocked the germination process. These results indicate that protein kinases of spores form complex networks sharing common modulating site that plays an important role in proper timing of early developmental events.

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Abbreviations

CDK:

cyclin-dependent protein kinase

DTT:

1,4-dithiothreitol

MAPK:

mitogen-activated protein kinase

MDC:

2,5-dihydroxycinnamic acid methyl ester

MLCK:

myosin-light chain kinase

mTOR:

mammalian target of rapamycin

PAGE:

polyacrylamide gel electrophoresis

PI3-kinase:

phosphatidylinositol 3-kinase

PK(s):

protein kinase(s)

PKA:

protein kinase A

PKC:

protein kinase C

PKG:

protein kinase G

PKI(s):

protein kinase inhibitor(s)

PTK:

protein tyrosine kinase

ROS:

reactive oxygen species

SDS:

sodium dodecylsulfate

SEM:

scanning electron microscopy

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Authors and Affiliations

  1. Institute of Microbiology, Academy of Sciences of the Czech Republic, 142 20, Prague, Czechia

    P. Palečková, F. Kontrová, O. Kofroňová, J. Bobek, O. Benada & K. Mikulík

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  1. P. Palečková
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  2. F. Kontrová
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  3. O. Kofroňová
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  4. J. Bobek
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  5. O. Benada
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  6. K. Mikulík
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Correspondence to K. Mikulík.

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This work was supported byGrant Agency of the Czech Republic grant no. 203/05/0106 to the corresponding author.

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Palečková, P., Kontrová, F., Kofroňová, O. et al. Effect of protein kinase inhibitors on protein phosphorylation and germination of aerial spores fromStreptomyces coelicolor . Folia Microbiol 52, 215–222 (2007). https://doi.org/10.1007/BF02931301

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  • DOI: https://doi.org/10.1007/BF02931301

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