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Regulation of proteins related to melanogenesis by heartwood extract ofMorus bombycis using proteome analysis

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Abstract

The ability of heartwood extract ofMorus bombycis in controlling the melanogenesis in B16 melanoma was investigated. An inhibitory effect was observed when the dose concentration was less than 10 mg/L. When the dose was 10 mg/L, the extracellular melanin accumulated was only 5% of the control. Proteome analysis with 2-D PAGE and MALDI-TOF showed that there were various proteins involved in inhibitory melanogenesis. It seems that down-regulation of TRP2, vacuolar protontranslocating ATPase 100 kDa subunit isoform a1-I and V-ATPase E2 subunit were involved in melanosome development and regulation that could reduce melanin synthesis.

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Authors and Affiliations

  1. Cosmetic R&D Center, COSMAX Inc., 445-746, Hwaseong, Korea

    Byoung-Sam Yoo

  2. Cosmetic Science Major, Applied Biotechnology Program, Graduate School, Ajou University, 443-749, Suwon, Korea

    Mi Ae Yoo & Sang Yo Byun

  3. Department of Molecular Science and Technology, Ajou University, 443-749, Suwon, Korea

    Young Keun Song & Sang Yo Byun

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  1. Byoung-Sam Yoo
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  2. Mi Ae Yoo
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  4. Sang Yo Byun
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Correspondence to Sang Yo Byun.

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Yoo, BS., Yoo, M.A., Song, Y.K. et al. Regulation of proteins related to melanogenesis by heartwood extract ofMorus bombycis using proteome analysis. Biotechnol. Bioprocess Eng. 12, 662–667 (2007). https://doi.org/10.1007/BF02931083

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  • DOI: https://doi.org/10.1007/BF02931083

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