Abstract
The ability of the yeastSaccharomyces cerevisiae to bioconvert stereo-selectively octyl-4-chloroacetoacetate (OCA) into the corresponding chiral alcohol, precursor of L-carnitin, an important physiological agent, was investigated.
In a monophasic system with free cells, more than 90% of OCA (0.018 M) bioconversion have been reached after 6 h (enantiomeric excess for the R form, eeR:97%). Immobilized cells in alginate beads were less efficient in conversion of OCA than free cells.
In a two-phase system with free cells, the level of reduction of OCA (0.018 M) reached 85% after 48 h. With a medium containing a higher OCA concentration (0.270 M ), 41% of this product were bioconverted after the same period. On the other hand, immobilized cells did not show any significant bioconversion of OCA in two-phase reactors. The limiting factor of these reactors is the regeneration of the cofactors involved in the OCA reduction.
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Bare, G., Jacques, P., Hubert, J.B. et al. Bioconversion of a L-carnitin precursor in a one- or two-phase system. Appl Biochem Biotechnol 28, 445–456 (1991). https://doi.org/10.1007/BF02922624
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DOI: https://doi.org/10.1007/BF02922624