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Effects of arachidonic acid concentration on prostaglandin biosynthesis and feasibility of semibatch processes

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Abstract

The reaction characteristics of prostaglandin E2 biosynthesis by PGH-synthase and PGE2 isomerase and the substrate dependency of this biosynthesis were studied. The activity of PG-synthases was blocked by the inhibitory action of one or more byproducts, probably resulting from the action of PGH-synthase. This inhibitory action then appeared to be partly reversible, indicating that the substrate and the inhibitor compete for the catalytic sites. According to these findings, the feasibility of a successful semibatch biosynthesis was investigated. A combination of the substrate concentration reducing procedure and the semibatch process resulted in an about 3.5-fold higher increase in the total amount of PGE2 formed in comparison with the batch results obtained at the substrate concentration of 1.0 mg/cm3. Since the cost of enzyme is a governing factor in this biosynthesis, development of semibatch biosynthesis of PGE2 becomes a matter of economic importance.

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Authors and Affiliations

  1. Department of Chemical Engineering, Kyoto University, 606, Sakyo-ku, Kyoto, Japan

    Eizo Sada, Shigeo Katoh & Azadeh Kheirolomoom

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  1. Eizo Sada
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  2. Shigeo Katoh
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  3. Azadeh Kheirolomoom
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Sada, E., Katoh, S. & Kheirolomoom, A. Effects of arachidonic acid concentration on prostaglandin biosynthesis and feasibility of semibatch processes. Appl Biochem Biotechnol 22, 247–261 (1989). https://doi.org/10.1007/BF02921760

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  • DOI: https://doi.org/10.1007/BF02921760

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