Abstract
Cell nuclei were isolated from leaf tissues of wild banana (Musa balbisiana, M. acuminata ssp.banksii andM. acuminata ssp.errans) and of the two vegetative clones of diploid cultivar “Pisang Mas”. Relative fluorescence intensity was measured on propidium iodide-stained nuclei by flow cytometry. Nuclei isolated fromGlycine max with known nuclear genome size were used as internal standard to determine nuclear DNA content ofMusa in absolute units. The results of the study showed that the size of nuclear genome ofMusa is smaller than previously estimated. In general, it is smaller in comparison with many other angiosperms. Furthermore, it was found that nuclear DNA content ofM. balbisiana (genome BB) is significantly lower than that ofM. acuminata subspecies and cultivars (genome AA). This finding should permit estimation of genome composition in triploidMusa clones with expected hybrid composition. Flow cytometry is proposed as a useful technique with potential applications in taxonomy, breeding and biotechnology ofMusa.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Afza, R., Kämer, D., Doležel, J., König, J., Van Duren, M., Kahl, G. Novák, F.J.: The potential of nuclear DNA flow cytometry and DNA fingerprinting forMusa improvement programmes.—In: Wills, B. (ed.): Proc. Int. Symp. Breeding Banana and Plantain for Resistance to Diseases and Pests. Pp. 65–75. CIRAD, Montpellier 1992.
Arumuganathan, K., Earle, E.D.: Nuclear DNA content of some important plant species.—Plant mol. Biol. Rep.9: 208–218, 1991.
Bennett, M.D., Smith, J.B.: Nuclear DNA amounts in angiosperms.—Phil. Trans. roy. Soc. LondonB 274: 227–274, 1976.
Bennett, M.D., Smith, J.B.: Nuclear DNA amounts in angiosperms.—Phil. Trans. roy. Soc. LondonB 334: 309–345, 1991.
Bennett, M.D., Smith, J.B., Heslop-Harrison, J.S.: Nuclear DNA amounts in angiosperms.—Proc. roy. Soc. London216: 179–199, 1982.
Crissman, H.A., Steinkamp, J.: Cytochemical techniques for multivariate analysis of DNA and other cellular constituents.—In: Melamed, M.R., Lindmo, T., Mendelsohn, M.L. (ed.): Flow Cytometry and Sorting. 2nd Edition. Pp. 227–247. Wiley-Liss, New York 1990.
Dean, P.N., Jett, J.H.: Mathematical analysis of DNA distributions derived from flow microfluorometry.—J. Cell Biol.60: 523–527, 1974.
De Laat, A.M.M., Göhde, W., Vogelzang, M.J.D.C.: Determination of ploidy of single plants and plant populations by flow cytometry.—Plant Breed.99: 303–307, 1987.
Dickson, E.E., Arumuganathan, K., Kresovich, S., Doyle, J.J.: Nuclear DNA content variation within theRosaceae.—Amer. J. Bot.79: 1081–1086, 1992.
Doležel, J.: FLOWSTAR: A microcomputer program for flow cytometric data manipulation and analysis.—Biológia (Bratislava)44: 287–291, 1989.
Doležel, J.: Flow cytometric analysis of nuclear DNA content in higher plants.—Phytochem. Anal.2: 143–154, 1991.
Doležel, J., Binarová, P., Lucretti, S.: Analysis of nuclear DNA content in plant cells by flow cytometry.—Biol. Plant.31: 113–120, 1989.
Doležel, J., Sgorbati, S., Lucretti, S.: Comparison of three DNA fluorochromes for flow cytometric estimation of nuclear DNA content in plants.—Physiol. Plant.85: 625–631, 1992.
Fox, M.H., Galbraith, D.W.: Application of flow cytometry and sorting to higher plant systems.—In: Melamed M.R., Lindmo T., Mendelsohn, M.L. (ed.): Flow Cytometry and Sorting. 2nd Edition. Pp. 633–650. Wiley-Liss, New York 1990.
Galbraith, D.W., Harkins, K.R., Maddox, J.M., Ayres, N.M., Sharma, D.P., Firoozabady, E.: Rapid flow cytometric analysis of the cell cycle in intact plant tissues.—Science220: 1049–1051, 1983.
Givan, A.L., Shenton, B.K., Carr, T.W.: A correction required for calculation of DNA ratios in flow cytometric analysis of ploidy.—Cytometry9: 271–274, 1988.
Hammatt, N., Blackhall, N.W., Davey, M.R.: Variation in the DNA content ofGlycine.—J. exp. Bot.42: 659–665, 1991.
Michaelson, M.J., Price, H.J., Ellison, J.R., Johnston, J.S.: Comparison of plant DNA contents determined by Feulgen microspectrophotometry and laser flow cytometry.—Amer. J. Bot.78: 183–188, 1991.
Moore, G., Gale, M.D., Kurata, N., Flavell, R.B.: Molecular analysis of small grain cereal genomes: current status and prospects.—Bio/Technology11: 584–589, 1993.
Sabharwal, P.S., Doležel, J.: Interspecific hybridization inBrassica: Application of flow cytometry for analysis of ploidy and genome composition in hybrid plants.—Biol. Plant.35: 169–177, 1993.
Simmonds, N.W., Shepherd, K.: The taxonomy and origins of the cultivated bananas.—J. Linnean Soc. Bot.55: 302–312, 1955.
Tiersch, T.R., Chandler, R.W., Wachtel, S.S.M., Elias, S.: Reference standards for flow cytometry and application in comparative studies of nuclear DNA content.—Cytometry10: 706–710, 1989.
Vindeløv, L.L., Christensen, I.J., Nissen, N.I.: Standardisation of high-resolution flow cytometric DNA analysis by the simultaneous use of chicken and trout red blood cells as internal reference standards.—Cytometry3: 328–331, 1983.
Author information
Authors and Affiliations
Additional information
Dedicated to the memory of Dr. F.J. Novák, who tragically died during preparation of this paper.
Rights and permissions
About this article
Cite this article
Doležel, J., Doleželová, M. & Novák, F.J. Flow cytometric estimation of nuclear DNA amount in diploid bananas (Musa acuminata andM. balbisiana). Biol Plant 36, 351–357 (1994). https://doi.org/10.1007/BF02920930
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02920930