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Zinc uptake by cultured human lymphoblasts

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Abstract

At physiological plasma concentrations (12–20 μM), zinc uptake by cultured human B lymphoblasts is biphasic with an early, rapid, saturable phase, followed by a slower, unsaturable phase. The saturable component is specific for zinc and cadmium, with aK m of 1–2 μM and aV max of 3.0 pmol/min/106 cells. This early phase does not require metabolic energy derived directly from ATP or the presence of sulfhydryl groups. At higher zinc concentrations (>30 μM), zinc uptake is more complex, although biphasic kinetics are also evident.

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Authors and Affiliations

  1. Department of Biology, University of California, San Diego, 92093, La Jolla, California

    Lois A. Eguchi & Paul Saltman

  2. Department of Medicine, University of California, San Diego, 92093, La Jolla, California

    Randall C. Willis & J. Edwin Seegmiller

Authors
  1. Lois A. Eguchi
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  2. Randall C. Willis
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  3. J. Edwin Seegmiller
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  4. Paul Saltman
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Eguchi, L.A., Willis, R.C., Seegmiller, J.E. et al. Zinc uptake by cultured human lymphoblasts. Biol Trace Elem Res 9, 177–186 (1986). https://doi.org/10.1007/BF02916525

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  • DOI: https://doi.org/10.1007/BF02916525

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