Abstract
Radiolabeled amino acid uptake assays are a highly sensitive method used to characterize the uptake of amino acids by cells or tissues in culture. This method is an excellent tool to quantify changes in amino acid consumption that are associated with states of cellular differentiation and/or disease. The methods presented here can be adapted to measure the transport of all amino acids and can be applied to cultured cells and bone explants.
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Acknowledgments
This work was supported by NIH/NIAMS grant AR071967 to C.M.K.
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Shen, L., Karner, C.M. (2021). Radiolabeled Amino Acid Uptake Assays in Primary Bone Cells and Bone Explants. In: Hilton, M.J. (eds) Skeletal Development and Repair. Methods in Molecular Biology, vol 2230. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1028-2_28
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DOI: https://doi.org/10.1007/978-1-0716-1028-2_28
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