Abstract
Eighty-one cultivated and 55 wild accessions were characterized using one- and two-dimensional isoelectric focusing (1D and 2D IEF)/SDS-PAGE and immuno-detection techniques to examine genetic diversity present within cultivated and wild taxa inVigna unguiculata (L.) Walp. Twenty-seven unique banding patterns were identified in the vignin (or Gl) fraction of the major seed storage proteins. These patterns were controlled by at least four interacting genes, two of which were tightly linked and a third which also may be linked. Due to the tremendous amount of variation in these wild taxa it is not possible to make definitive statements about either the taxonomic or geographic distribution of vignin types. These results give no final answer to the identity of the progenitor of cowpea or the center of domestication, but do pose some interesting questions to be asked as the genetic relationships among the wild and cultivated taxa in this species are unraveled.
Résumé
Quatre-vingt-un formes cultivées et 55 formes sauvages ont été analysées par électrophorèse en gel de polyacrylamide-SDS à une et deux dimensions et par immuno-detection afin d’examiner la diversité génétique de Vigna unguiculata (L.) Walp. Vingt-sept patrons uniques de bandes ont été identifiés dans la fraction de la vignine, la protéine principale de la sentence. Ces patrons sont contrôlés par au moins quatre gènes, deux desquels sont fortement liés tandis qu ’un troisième pourrait l’être. A cause du niveau de variation très élevé parmi les formes sauvages, il ne nous a pas été possible d’établir des conclusions fermes au sujet de la distribution géographique et taxonomique des différents types de vignine. Nos résultats n ’apportent pas de réponse définitive quant à l’identité des formes ancestrales du niébé et la localisation du ou des centres de domestication; en revenche, Us permettent de poser d’intéressantes questions sur les relations entre formes sauvages et cultivées de cette espèce afin de guider des recherches futures.
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Panella, L., Kami, J. & Gepts, P. Vignin diversity in wild and cultivated taxa ofVigna unguiculata (L.) walp. (fabaceae). Econ Bot 47, 371–386 (1993). https://doi.org/10.1007/BF02907351
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DOI: https://doi.org/10.1007/BF02907351