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Synergistic effects of cellobiose dehydrogenase and manganese-dependent peroxidases during lignin degradation

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Chinese Science Bulletin

Abstract

The synergistic effects of cellobiose dehydrogenase (CDH) and manganese-dependent peroxidases (MnP) on the degradation of kraft pulp cellulolytic enzyme lignin (CEL) were investigated. Addition of CDH significantly increased the amount of water-soluble products reduced from CEL by MnP. CDH facilitated the reduction of the contents of methoxyl, carboxyl, phenolic hydroxyl and total hydroxyl groups of CEL by MnP.1H-NMR analysis showed that addition of CDH also decreased further the amount of protons of CEL degraded by MnP. The results proved for the first time that CDH could promote degradation of lignin by MnP and suggest that CDH could not only promote degradation of cellulose but also is an important part of the lignin biodegradation system.

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Authors and Affiliations

  1. State Key Laboratory of Microbial Technology, Shandong University, 250100, Jinan, China

    Feng Huang, Jing Fang, Xuemei Lu & Peiji Gao

  2. State Key Lab of Pulp and Paper Engineering, South China University of Technology, 510641, Guangzhou, China

    Jiaxiang Chen

Authors
  1. Feng Huang
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  2. Jing Fang
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  3. Xuemei Lu
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  4. Peiji Gao
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  5. Jiaxiang Chen
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Correspondence to Peiji Gao.

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Huang, F., Fang, J., Lu, X. et al. Synergistic effects of cellobiose dehydrogenase and manganese-dependent peroxidases during lignin degradation. Chin.Sci.Bull. 46, 1956–1961 (2001). https://doi.org/10.1007/BF02901905

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  • DOI: https://doi.org/10.1007/BF02901905

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