Skip to main content
SpringerLink
Log in

Transgenic rice homozygous lines expressing GNA showed enhanced resistance to rice brown planthopper

  • Notes
  • Published:
Chinese Science Bulletin

Abstract

Mature seed-derived calli from two elite Chinese japonica rice (Oryza sativa L.) cultivars Eyi 105 and Ewan 5 were co-transformed with two plasmids, pWRG1515 and pRSSGNA1, containing the selectable marker hygromycin phosphotransferase gene (hpt), the reporter β-glucuronidase gene (gusA) and the snowdrop (Galanthus nivalis) lectin gene (gna) via particle bombardment. 61 independent transgenic rice plants were regenerated from 329 bombarded calli. 79% transgenic plants contained all the three genes, revealed by PCR/Southern blot analysis. Western blot analysis revealed that 36 out of 48 gna-containing transgenic plants expressed GNA (75%) at various levels with the highest expression being approximately 0.5% of total soluble protein. Genetic analysis confirmed Mendelian segregation of transgenes in progeny. From the R2 generations whose R1 parent plants showing 3:1 Mendelian segregation patterns, we identified five independent homozygous lines containing and expressing all the three transgenes. Insect bioassay and feeding tests showed that these homozygous lines had significant inhibition to rice brown planthopper (Nilaparvata lugens, BPH) by decreasing BPH survival and overall fecundity, retarding BPH development and declining BPH feeding. These BPH-resistant lines have been incorporated into rice insect resistance breeding program. This is the first report that homozygous transgenic rice lines expressing GNA, developed by genetic transformation and through genetic analysis-based selection, conferred enhanced resistance to BPH, one of the most damaging insect pests in rice.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Khus, G. S., Brar, D. S., Genetics of resistance to insects in crop plants, Adv. Agro., 1991, 45: 223.

    Google Scholar 

  2. Brar, D. S., Khush, G. S., Alien introgression in rice., Plant Mol. Biol., 1997, 35: 35.

    Article  PubMed  CAS  Google Scholar 

  3. Cheng, X., Sardana, R., Kaplan, H. et al., Agrobacterium-transformed rice plants expressing synthetic cryIA(b) and cryIA(c) genes are highly toxic to striped stem borer and yellow stem borer, Proc. Natl. Acad. Sci. USA, 1998, 95: 2767.

    Article  PubMed  CAS  Google Scholar 

  4. Song, W. Y., Wang, G. L., Chen, L. L. et al., A receptor kinase-like protein encoded by the rice disease resistance gene, Xa21, Science, 1995, 270: 1804.

    Article  PubMed  CAS  Google Scholar 

  5. Mochida, O., Wahyu, A., Surjani, T. K., Some considerations on screening resistant cultivars/lines of rice plant to the brown planthopper, Nilparvata Lugens (Stal), (Hom., Delphacidae), IRRI, 1979, 1.

  6. Powell, K. S., Gatehouse, A. M. R., Hilder, V. A. et al., Antimetabolic effects of plant lectins and plant and fungal enzymes on the nymphal stages of two important rice pest, Nilaparvata lugens and Nephotettix cinciteps, Entomol. Exp. Appl., 1993, 66: 119.

    Article  CAS  Google Scholar 

  7. Powell, K. S., Gatehouse, A. M. R., Hilder, V. A. et al., Antifeedant effects of plant lectins and an enzymes on the adult stage of the rice brown planthopper, Nilaparvata lugens, Entomol. Exp. Appl., 1995, 75: 51.

    Article  CAS  Google Scholar 

  8. Pusztai, A., Ewan, S. W. B., Grant, G. et al., The relationship between survival and binding of plant lectins during small intestinal passage and their effectiveness as growth factors, Digest., 1990, 46: 308

    Article  CAS  Google Scholar 

  9. Sudhakar, D., Fu, X., Stoger, E. et al., Expression and immunolocalization of the snowdrop lectin insecticidal protein GNA in transgenic rice plants, Transgenic Res., 1998, 7: 371.

    Article  PubMed  CAS  Google Scholar 

  10. Tang, K., Tinjuangjun, P., Xu, Y. et al., Particle bombardment-mediated co-transformation of elite Chinese rice cultivars with genes conferring resistance to bacterial blight an sap sucking insect pests, Planta, 1999, 208: 552.

    Article  CAS  Google Scholar 

  11. Rao, K. V., Rathore, K. S., Hodges, T. K. et al., Expression of snowdrop lectin (GNA) in transgenic rice plants confers resistance to rice brown planthopper, Plant. J, 1998, 15: 469.

    Article  PubMed  CAS  Google Scholar 

  12. Christou, P., Ford, T. L., Kofron, M., Production of transgenic rice (Oryza sativa L.) plants from agronomically important indica and japonica varieties via electric-discharge particle-acceleration of exogenous DNA into immature zygotic embryos, Bio/Technol, 1991, 9: 957.

    Article  Google Scholar 

  13. Wang, M. B., Boulter, D., Gatehouse, J. A., A complete sequence of the rice sucrose synthase-1 (Rss1) gene, Plant Mol. Biol., 1992, 19: 881.

    Article  PubMed  CAS  Google Scholar 

  14. Edwards, K., Johmstone, C., Thompson, C., A simple and rapid method for the preparation of plant genomic DNA for PCR analyses, Nucl. Acids. Res., 1991, 98: 1349.

    Article  Google Scholar 

  15. Jefferson, R. A., Kavanagh, T. A., Bevan, M. W., GUS fusions: β-glucuronidase as a sensitive and versatile gene fusion marker in higherplants, EMBO J., 1987, 6: 3901.

    PubMed  CAS  Google Scholar 

  16. Christou, P., Strategies for variety-independent genetic transformation of important cereals, legumes and woody species utilizing particle bombardment, Euphytica, 1995, 85: 13.

    Article  Google Scholar 

  17. Hiei, Y., Ohta, S., Komari, T. et al., Efficient transformation of rice (Oryza sativa L.) mediated by Agrobacterium and sequence analysis of the boundaries of the T-DNA, Plant. J., 1994, 6: 271.

    Article  PubMed  CAS  Google Scholar 

  18. Chen, L., Marmey, P., Taylor, N. J. et al., Expression and inheritance of multiple transgenes in rice plants, Nature Biotechnol, 1998, 16: 1060.

    Article  CAS  Google Scholar 

  19. Cooley, J., Ford, T., Christou, P., Molecular and genetic characterization of elite transgenic rice plants produced by electric discharge particle acceleration, Theor. Appl. Genet., 1995, 90: 97.

    Article  CAS  Google Scholar 

  20. Kohli, A., Leech, M., Vain, P. et al., Transgene organization in rice engineered through direct DNA transfer supports a two-phase integration mechanism mediated by the establishment of integration hot-spots, Proc. Natl. Acad. Sci. USA, 1998, 95: 7203.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, 200433, Shanghai, China

    Sun Xiaofen & Tang Kexuan

  2. Hybrid Rice Research Center, Hubei Academy of Agricultural Sciences, 430064, Wuhan, China

    Wan Bingliang, Qi Huaxiong & Lu Xinggui

Authors
  1. Sun Xiaofen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Tang Kexuan
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Wan Bingliang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Qi Huaxiong
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Lu Xinggui
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Tang Kexuan.

About this article

Cite this article

Xiaofen, S., Kexuan, T., Bingliang, W. et al. Transgenic rice homozygous lines expressing GNA showed enhanced resistance to rice brown planthopper. Chin. Sci. Bull. 46, 1698–1703 (2001). https://doi.org/10.1007/BF02900655

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02900655

Keywords

Search

Navigation