Abstract
Using a specific radioimmunoassay for gonadotropin releasing hormone, the presence of gonadotropin releasing hormone like material in the first trimester human placenta has been demonstrated. The material has been partially characterized using carboxy methyl cellulose chromatography, high pressure gel permeation chromatography and reverse phase C18 high pressure liquid chromatographic analysis. Analysis for bioactivity revealed that placental gonadotropin releasing hormone is much more active than synthetic gonadotropin releasing hormone inin vitro rat pituitary lutinising hormone release assay.In vitro biosynthetic studies using labelled precursors and immunoaffinity chromatography indicated that first trimester human placenta synthesizes gonadotropin releasing hormone like material.
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Abbreviations
- GnRH:
-
Gonadotropin releasing hormone
- CG:
-
chorionic gonadotropin
- IgG:
-
immunoglobulin G
- CM:
-
cellulose carboxy methyl cellulose
- BSA:
-
bovine serum albumin
- EMEM:
-
Eagle’s minimum essential medium
- EBSS:
-
Earle’s balanced salt solution
- HP-GPC:
-
high pressure gel permeation chromotography
- HPLC:
-
higher pressure liquid chromatography
- LH:
-
lutinising hormone
- HCG:
-
human chorionic gonadotropin
- CNBr:
-
cyanogen bromide
- RP C 18:
-
reverse phase C 18
- RIA:
-
radioimmunoassay
- GAP:
-
GnRH associated peptide
References
Akanxuma, Y. and Hayashi, M. (1974)Methods Enzymol.,34, 746.
Aten, R. F., Williams, A. T. and Behrman, H. R. (1986)Endocrinology,118, 961.
Das, C. and Talwar, G. P. (1983)Fertil. Steril.,39, 218.
Elkind-Hirsch, K., Ravniker, V., Schiff, I., Tulchinsky, D. and Ryan, K. J. (1982)J. Clin. Endocrinol. Metab.,54, 602.
Gautron, J P, Pattou E and Kordon C (1981)Mol CellEndocrinol 24 1
Gibbons, J. M., Mitnick, M. and Chieffo, V. (1975)Am. J. Obstet. Gynecosl.,121, 127.
Greenwood, F. C., Hunter, N. M. and Glover, J. S. (1963)Biochem. J.,89, 114.
Guillemin, R., Jutisz, M. and Sakiz (1963)C.R. Acad. Sci. 256, 504.
Hedger, M. P., Robertson, D. M., Browne, C. A. and de Kretser, D. M. (1985)Mol. Cell. Endocrinol.,42, 163.
Hsueh, A. J. W. and Jones, P. B. C. (1982) inInterovarian Control Mechanisms (eds C. P. Channing and S. J. Segal) (New York: Plenum Press) p. 223.
Khodr, G. S. and Siler-Khodr, T. M. (1978)Fertil. Steril.,30, 301.
Khodr, G. S. and Siler-Khodr, T. M. (1980)Science,207, 315.
Koch, Y., Baram, T., Chobsieng, P. and Fridkin, M. (1974)Biochem. Biophys. Res. Commun.,61, 95.
Malik, A., Kaul, S. and Das, C. (1986)J. Biosci.,10, 67.
Mathialagan, N. and Rao, A. J. (1986)J. Biosci.,10, 423.
Nikolics, K., Szoke, B., Keri, G. and Teplan, I. (1983)Biochem. Biophys. Res. Commun. 114, 1028.
Nikolics, K., Mason, A. J., Szonyi, E., Ramachandran, J. and Seeberg, P. H. (1985)Nature (London),316, 511.
Nowak, R. A., Wiseman, S. B. and Bahr, J. M. (1984)Biol. Reprod.,31, 67.
Rao, A. J., Mathialagan, N., Kotagi, S. G. and Moudgal, N. R. (1984)J. Biosci. (Suppl. 2),6, 97.
Rao, A. J. and Mathialagan, N. (1985)Biol. Reprod. (Suppl. 1),32, 150, Abstract 225.
Sairam, M. R., Ranganathan, M. R., Ramasharma, K. and Lamothe, P. (1981)Mol. Cell. Endocrinol.,22, 231.
Seeberg, P. H. and Adelman, J. P. (1984)Nature (London),311, 666.
Siler-Khodr, T. M., Khodr, G. S., Valenzuela, G. and Rhode, J. (1986)Biol. Reprod.,34, 245.
Tan, L. and Rousseau, P. (1982)Biochem. Biophys. Res. Commun.,109, 1061.
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Mathialagan, N., Rao, A.J. Gonadotropin releasing hormone in first trimester human placenta: Isolation, partial characterisation andin vitro biosynthesis. J. Biosci. 10, 429–441 (1986). https://doi.org/10.1007/BF02900503
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DOI: https://doi.org/10.1007/BF02900503