Abstract
The effect of fungicides on the production of aflatoxin byAspergillus flavus IMI 89717, diacetoxyscirpenol and zearalenone byFusarium graminearum was studied. In a yeast extract - sucrose medium, dicloran, iprodione and vinclozolin fungicides significantly inhibited mycelial growth ofA. flavus at 250 ppm and significantly decreased aflatoxin production at 100, 250 and 500 ppm, respectively. In potato — dextrose broth, these fungicides diminished the mycelial growth ofF. graminearum and production of diacetoxyscirpenol and zearalenone at 100 ppm. Sensitivity of toxigenic mycelia to fungicides increased approximately five-fold in a yeast extract-starch medium with an appreciable reduction in sugar uptake andα-amylase activity.
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References
Draugnon F.A., Churchville D.C.: Effect of pesticides on zearalenone production in culture and in corn plants.Phytopathology 75, 553–556 (1985).
Draughon F.A., Elahi M.E., West D.R.: Insecticide inhibition of aflatoxin production in corn.J.Agric.Food Chem. 31, 692–695 (1983).
Hasan H.A.H.: Selective effect of some pesticides on mycoflora and mycotoxins production in corn grains and sunflower seeds.MSc Thesis. Faculty of Science, Assiut University (Egypt) 1988.
Kaars Sipesteijn A.: Mechanism of action of fungicides, pp. 32–45 in I. Dekker, S.G. Georgopoulos (Eds):Fungicide Resistance in Crop Protection. Centre for Agricult. Publ. and Document., Wageningen 1982.
Leroux P., Fritz R.: Antifungal activity of dicarboximides and aromatic hydrocarbons and resistance to these fungicides, pp. 207–237 in A.P.J. Trinci, J.F. Ryley (Eds):Mode of Action of Antifungal Agents. Brit. Mycol. Soc. 1984.
Leroux P., Fritz R., Gredt M.: Etudes en laboratoire de souches deBotrytis cinerea Pers., resistances a la dichlozoline, au dicloran, au quintozene, a la vinclozoline et au 26019 RP (on glycophene).Phytopath.Z. 89, 347 (1977).
Mirocha C.J., Schauerhamer B., Pathre S.V.: Isolation, detection and quantitation of zearalenone in maize and barley.J.Assoc.Off.Anal.Chem. 57, 1104–1110 (1974).
Nabney J., Nesbitt B.F.: A spectrophotometric method for determining the aflatoxins.Analyst 90, 155–160 (1965).
Naguib M.I.: Effect of ascorbic acid on respiration and carbohydrate metabolism ofCunninghamella sp.Folia Microbiol. 10, 215–223 (1965).
Nelson N.: A photometric adaptation of the Somogyi method for determination of glucose.J.Biol.Chem. 153, 375–380 (1944).
Passi S., Nazzaro-Porro M., Picardo M., Finotti E., Fabbri A.A., Fanelli C.: Microsomal and mitochondrial involvement in production of aflatoxins induced by carbon tetrachloride and hydroperoxide in cultures ofAspergillus parasiticus.Trans.Brit.Mycol.Soc. 87, 451–456 (1986).
Ritchie D.F.: Effect of Dichloran, Iprodione, Procymidone and Vinclozolin on the mycelial growth, sporulation and isolation of resistant strains ofMonilinia fructicola.Plant Dis. 66, 484–486 (1982).
Scott P.M., Lawrence J.W., Van Walbeak W.: Detection of mycotoxins by thin-layer chromatography: Application to screening of fungal extracts.Appl.Microbiol. 20, 839–842 (1970).
Takitani S., Asabe U., Kato T., Suzuki M., Ueno Y.: Spectrodensitometric determination of trichothecene-mycotoxins with 4-(p-nitrobenzyl)pyridine on silica gel thin-layer chromatograms.J.Chromatog. 172, 335–342 (1979).
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Hasan, H.A.H. Fungicide inhibition of aflatoxins, diacetoxyscirpenol and zearalenone production. Folia Microbiol 38, 295–298 (1993). https://doi.org/10.1007/BF02898597
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DOI: https://doi.org/10.1007/BF02898597