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Effects of cadmiumin vitro on contractile and relaxant responses of isolated rat aortas

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Environmental Health and Preventive Medicine Aims and scope

Abstract

Objective

Cadmium is known to affect the vascular tone of isolated blood vesselsin vitro and the arterial pressure of ratsin vivo. However, the mechanisms of cadmium actions on the vascular system have not been clarified. To elucidate the actions of cadmium on vascular tonus, effects of cadmium on vasocontractile and vasorelaxant responsesin vitro were investigated using aortic strips isolated from rats.

Methods

Aortic strips isolated from male Wistar rats were incubated with CdCl2 (10μM) for 24 hr, washed with fresh CdCl2-free medium, and then used for measurement of isometric tension and Western blot analysis of eNOS (endothelial nitric oxide synthase) and iNOS (inducible nitric oxide synthase).

Results

In the aortas pretreated with cadmiumin vitro, the contractile response to phenylephrine was significantly higher than that in the control aortic strips pretreated with a vehicle. The sodium nitroprusside-induced relaxing response was significantly higher in the aortic strips pretreated with cadmium for 24 hr, compared with that in the control pretreated with a vehicle. The isoproterenol-induced relaxing response was also significantly higher in the cadmium-accumulated aortic strips.In vitro cadmium treatment slightly but not significantly increased the acetylcholine-induced relaxation of the aortic strips. Cadmium treatment induced expression of iNOS and significantly increased expression of eNOS in the aortic strips, while it did not affect expression of β-actin.

Conclusions

Cadmium treatmentin vitro augmented the α1 adrenoceptor-mediated contractile response, even though eNOS and iNOS were upregulated by cadmium treatment. NO-induced and β-adrenoceptor-mediated relaxing responses were also augmented by cadmium treatment. These results suggest that both vasocontractile and vasorelaxing responses are augmented in cadmium-accumulated aortas.

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Authors and Affiliations

  1. Department of Hygiene & Preventive Medicine, Yamagata University School of Medicine, Iida-Nishi2-2, 990-9585, Yamagata, Japan

    Yuji Takahashi, Michael Poteser & Ichiro Wakabayashi

  2. Department of Public Health, Hyogo College of Medicine, Nishinomiya, Japan

    Hidehisa Masui & Naoko Koizumi

Authors
  1. Yuji Takahashi
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  2. Michael Poteser
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  3. Hidehisa Masui
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  4. Naoko Koizumi
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  5. Ichiro Wakabayashi
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Correspondence to Yuji Takahashi.

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Takahashi, Y., Poteser, M., Masui, H. et al. Effects of cadmiumin vitro on contractile and relaxant responses of isolated rat aortas. Environ Health Prev Med 9, 251–256 (2004). https://doi.org/10.1007/BF02898138

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  • DOI: https://doi.org/10.1007/BF02898138

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