Summary
To investigate the significance of hepatocyte apoptosis, proliferation and oncogene c-fos expression in carbon tetrachloride (CCl4)-induced cirrhotic rat liver. Rat cirrhosis was induced by subcutaneous injection of 50% (v:v 1:1) CCl4. Hepatocyte apoptosis, proliferation and oncogene c-fos expression were examined with TUNEL, PCNA and c-fos immunohistochemical methods in control group and treatment group 72 h, 5, 7, 11 and 15 weeks after CCl4, induction. Hepatocyte apoptosis was rarely seen in control rat liver. The hepatoeyte apoptosis was obviously increased 72 h after treatment. Fifteen weeks after treatment, the apoptosis was still more obvious in treatment group than that in controls. PCNA was constantly expressed in CCl4 group, with highest level at middle phase. C-fos was positive 7 and 11 weeks after CCl4 treatment. The results suggest that: 1) apoptosis is involved in rat liver damage at the early phase in CCl4-induced injury, and the process can alleviate nodule reconstruction or eradicate potentially mutational hepatocytes at the later phase; 2) hepatocytes constantly proliferate in CCl4-induced rat liver cirrhosis, especially at the middle phase; 3) c-fos might modulate hepatocyte proliferation in CCl4-induced rat liver cirrhosis.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Tushar P, Gores G J. Apotosis and hepatobiliary diseases. Hepatol, 1995, 21(6): 1725
Benz C, Angermuller S, Kloters-Plachky Pet al. Effect of S-adenosylmethionine versus tauroursodeoxycholic acid on bile acid-induced apoptosis and cytolysis in rat hepatocytes. Eur J Clin Invest, 1998, 28(7):577
1984, 1(4):145
Leist M, Gantner F, Bohlinger Iet al. The bcl-2 protooncogene and the gastrointestinal epithelial tumor progression model. Am J Pathol, 1995, 146(1):1220
Delhaye M, Louis H, Degraef Cet al. Relationship between hepatocyte proliferation activity and liver functional reserve in human cirrhosis. Hepatol, 1996, 23(5): 1003
Nagy P, Bisgaard H G, Rugin E Set al. In vivo infusion of growth factors enhances the mitogenic response of the hepatic ductal cells after administration of 2-acetylaminofluorene. Hepatol, 1996, 23(1):840
Nagoshi S, Yasuda H, Suda Jet al. Hepatocyte apoptosis and hepatic expression of transforming growth factor-beta 1 mRNA during involution of hyperplastic rat liver induced by hepatocyte growth factor. J Gastroenterol Hepatol, 1998, 13(8):786
Ewico A O, Fioremtino M, Ponzetto Aet al. Liver hepatocyte growth factor does not always correlate with hepatocellular proliferation in human liver lesions: its specific receptor c-met does. Hepatol, 1996, 24(1): 60
Ogasawara J, Watanabeo F R, Adachi Met al. Lethal effect of the antiFAS antibody in mice. Nature, 1993, 364:806
Collota F, Pollentarutti N, Sironi Met al. Expression and involvement of c-fos and c-jun protooncogenes in programmed cell death induced by growth factors deprivation in lymphoid cell lines. J Biol Chem, 1992, 267:18–278
Ferrer I, Segui J, Olive M. Strong c-Jun immunoreactivity is associated with apoptotic cell death in human tumors of the central nervous system. Neurosci Lett, 1996, 16,214(1):49
Bruccoleri A, Gallucci R, Germolec D Ret al. Induction of early-immediate genes by tumor necrosis factor alpha contribute to liver repair following chemical-induced hepatotoxicity. Hepatology, 1997, 25(1): 133
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Libo, C., Zhen, Y. & Fazu, Q. Studies on hepatocyte apoptosis, proliferation and oncogene c-fos expression in carbon tetrachloride-induced cirrhotic rat liver. Journal of Tongji Medical University 19, 53–55 (1999). https://doi.org/10.1007/BF02895597
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02895597