Summary
Comparative immunofluorescence microscopic, transmission and scanning electron microscopic investigations were carried out to study the arrangement and significance of vimentin filaments in monocytes, macrophages, epithelioid cell equivalents and multinucleate giant cells under various different functional conditions, and in the presence of functional disorders.
Uncoated or sebum-coated coverslips were implanted in the peritoneal cavity of Wistar rats. Some of the animals received repeated i.p. injections of colchicine. Rats were killed at various times 1 to 14 days after initiation of the experiment. The number of macrophages, the degree of their activation, and the growth of cells on the coverslips was considerably greater on sebum-coated than on uncoated implants. Various characteristic vimentin distribution patterns were found dependent on the cell cycle, the form and volume of the cell, and on the degree of differentiation and maturity; they were also related to the type and intensity of cell function. These patterns were best developed in ordered multinucleate giant cells.
Repeated administrations of colchicine resulted in a marked flattening of the cell body on the coverslips — which correlated with a considerable reduction in the number of vimentin filaments and of cytoplasmic processes — and also in the formation of circumscribed erect, tree-like protuberances. The “trunk” of these structures comprised closely bundled vimentin filaments, and the cell nucleus was located at its base. These morphologic changes, which were associated with a functional insufficiency, proved to be reversible.
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References
Aubin JE, Osborn M, Franke WW, Weber K (1980) Intermediate filaments of the vimentin type and the cytokeratin type are distributed differently during mitosis. Exp Cell Res 129:149–165
Berlin RD, Oliver JM (1978) Analogous ultrastructure and surface properties during capping and phagocytosis in leukocytes. J Cell Biol 77:789–804
Blose SH, Chacko S (1976) Rings of intermediate (100 Å) filament bundles in the perinuclear region of vascular endothelial cells: their mobilization by colcemid and mitosis. J Cell Biol 70:459–466
Cain H (1981) Granulome und Granulomatosen. Eine zellbiologische und zellularpathologische Betrachtung. Pathologe 2:65–71
Cain H, Kraus B (1980) Mehrkernige Riesenzellen in Granulomen. Neuordnung der Binnenstruktur nach Konfluenz von Zellen des Makrophagensystems. Virchows Arch [Pathol Anat] 385:309–333
Cain H, Kraus B (1981) Cytoskeleton in cells of the mononuclear phagocyte system. Immunofluorescence microscopic and electron microscopic studies. Virchows Arch [Cell Pathol] 36:159–176
Cain H, Kraus B, Fringes B, Osborn M, Weber K (1981) Centrioles, microtubules and microfilaments in activated mononuclear and multinucleate macrophages from rat peritoneum: electron microscopic and immunofluorescence microscopic studies. J Pathol 133:301–323
Farquhar MG, Palade GE (1981) The Golgi apparatus (complex) — (1954–1981) — from artifact to center stage. J Cell Biol 91:77s-103s
Franke WW, Schmid E, Osborn M, Weber K (1978) Different intermediate-sized filaments distinguished by immunofluorescence microscopy. Proc Natl Acad Sci USA 75:5034–5038
Franke WW, Schmid E, Winter S, Osborn M, Weber K (1979) Widespread occurrence of intermediate-sized filaments of the vimentin-type in cultured cells from diverse vertebrates. Exp Cell Res 123:25–46
Gawlitta W, Osborn M, Weber K (1981) Coiling of intermediate filaments induced by microinjection of a vimentin-specific antibody does not interfere with locomotion and mitosis. Eur J Cell Biol 26:83–90
Kraus B (1980) Mehrkernige Riesenzellen in Granulomen. Verh Dtsch Ges Pathol 64:103–125
Kraus B (1982) Formation of giant cells in vivo. Immunobiol 161:290–297
Malawista SE, Oliver JM, Rudolph SA (1978) Microtubules and cyclic AMP in human leukocytes: on the order of things. J Cell Biol 77:881–886
Malech HL, Root RK, Gallin JI (1977) Structural analysis of human neutrophil migration. Centriole, microtubule and microfilamental orientation and function during chemotaxis. J Cell Biol 75:666–693
Melmed RN, Karanian PJ, Berlin RD (1981) Control of cell volume in the J 774 macrophage by microtubule disassembly and cyclic AMP. J Cell Biol 90:761–768
Oliver JM, Albertini DT, Berlin RD (1976) Effects of gluthatione-oxidizing agents on microtubule assembly and microtubule-dependent surface properties of human neutrophils. J Cell Biol 71:921–932
Osborn M, Weber K (1982) Immunofluorescence and immunocytochemical procedures with affinity-purified antibodies. In: Wilson L (ed), Methods in cell biology. Academic Press, London-New York (in press)
Sutton JS, Weiss L (1966) Transformation of monocytes in tissue cultures into macrophages, epithelioid cells and multinucleate giant cells. J Cell Biol 28:303–332
Walter RJ, Berlin RD, Pfeiffer JR, Oliver JM (1980) Polarization of endocytosis and receptor topography on cultured macrophages. J Cell Biol 86:199–211
Wang E, Cross RK, Choppin PW (1979) Involvement of microtubules and 10 nm filaments in the movement and positioning of nuclei in syncytia. J Cell Biol 83:320–337
Weber K, Osborn M (1982) Microtubules and intermediate filament networks in cells viewed by immunofluorescence microscopy. In: Post G, Nicolson GI (eds.) Cell surface reviews. Biomedical Press, Amsterdam-New York (in press)
Weber K, Bibring T, Osborn M (1975) Specific visualization of tubulin containing structures in tissue culture cells by immunofluorescence. Exp Cell Res 95:111–120
Zieve GW, Heidemann SR, Mclntosh JR (1980) Isolation and partial characterization of a cage of filaments that surrounds the mammalian mitotic spindle. J Cell Biol 87:160–169
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Cain, H., Kraus, B., Krauspe, R. et al. Vimentin filaments in peritoneal macrophages at various stages of differentiation and with altered function. Virchows Archiv B Cell Pathol 42, 65–81 (1983). https://doi.org/10.1007/BF02890371
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DOI: https://doi.org/10.1007/BF02890371