Summary
Keratin expression in hamster tracheal epithelium was investigated during organ culture in serum-free, hormone-supplemented medium using monospecific monoclonal antibodies. Generally, tracheal basal cells expressed keratins detected by antibodies RCK102 and RCK103, while columnar epithelial cells were stained positively by RGE53, RCK103, RCK105 and HCK19. Metaplastic squamous cell foci reacted with antibodies RKSE60, RCK103 and HCK19. Early metaplastic alterations were more clearly RKSE60-positive than the mature lesions. In the vitamin A-depleted tracheas basal cells were clearly RCK102-positive. Superficial cells in the central part of areas of squamous metaplasia induced by cigarette smoke condensate expressed the basal cell keratins, and were negative for the columnar cell keratin 18 detected by the RGE53 antibody. This finding suggests that in cigarette smoke condensate-induced squamous metaplasia basal cells play an important role. The mucus-producing cells at the edges of metaplastic squamous cell foci expressed the keratins specific to columnar cells. Cigarette smoke condensate exposure accelerated epithelial keratinization compared to the vitamin A-depleted epithelium. It was concluded that not only small mucous granule cells, but also basal cells are involved in the development and maintenance of induced squamous metaplasia in tracheal epithelium. Furthermore, in vitro vitamin A-depleted epithelium did not coexpress vimentin in addition to the different keratins.
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References
Bock FG, Clausen DF (1980) Further fractioriation and copromoting activity of the large molecular weight components of aqueous tobacco extracts. Carcinogenesis 1:317–321
Chopra DP (1982) Squamous metaplasia in organ cultures of vitamin A-deficient hamster trachea: Cytokinetic and ultrastructural alterations. J Natl Cancer Inst 69:895–905
Chopra DP (1983) Cell dynamics in expiants derived from tracheas of hamsters fed normal and vitamin A-deficient diets. Cell Tissue Kinet 16:155–165
Chopra DP, Cooney RA (1985) Histogenesis of benzo[a]pyreneinduced lesions in tracheal explants. Virchows Arch [Cell Pathol] 48:299–315
Clamon GH, Sporn MB, Smith JM, Saffiotti U (1974) α- and β-retinyl acetate reverse metaplasias of vitamin A deficiency in hamster trachea in organ culture. Nature 250:64–66
De Luca LM, Roop D, Huang FL (1985) Vitamin A: A key nutrient for the maintenance of epithelial differentiation. Acta Vitaminol Enzymol 7, 13–20
De Marini DM (1983) Genotoxicity of tobacco smoke and smoke condensate. Mutat Res 114:59–89
Gellhorn A (1958) Co-carcinogenic activity of cigarette tobacco tar. Cancer Res 18:510–517
Huang FL, Roop D, DeLuca LM (1986) Vitamin A deficiency and keratin biosynthesis in cultured hamster trachea. In Vitro 22, 223–230
IARC, International Agency for Research on Cancer (1986) IARC monographs on the evaluation of the carcinogenic risk of chemicals to humans, vol 38. Tobacco Smoking, Lyon, France
Kopan R, Traska G, Fuchs E (1987) Differences in keratin synthesis between normal epithelial cells and squamous cell carcinomas are mediated by vitamin A. Proc Natl Acad Sci 81:4280–4284
Lasnitzki I (1958) Observations on the effects of condensates from cigarette smoke on human foetal lung in vitro. Br J Cancer 12:547–552
Lasnitzki I (1968) The effect of a hydrocarbon-enriched fraction from cigarette smoke on mouse tracheas grown in vitro. Br J Cancer 22:105–109
Lasnitzki I, Bollag W (1982) Prevention and reversal by a retinoid of 3,4-benzpyrene-and cigarette smoke condensate-induced hyperplasia and metaplasia of rodent respiratory epithelia in organ culture. Cancer Treat Rep 66:1375–1380
Lasnitzki I, Bollag W (1987) Prevention and reversal by a nonpolar arotinoid (Ro 15-0778) of 3,4-benzpyrene-and cigarette smoke condensate-induced hyperplasia and metaplasia of rodent respiratory epithelia grown in vitro. Eur J Cancer Clin Oncol 23:861–865
McDowell EM, Keenan KP, Huang M (1984a) Effects of vitamin A-deprivation on hamster tracheal epithelium. A quantitative morphologic study. Virchows Arch [Cell Pathol] 45:197–219
McDowell EM, Keenan KP, Huang M (1984b) Restoration of mucociliary tracheal epithelium following deprivation of vitamin A. A quantitative morphologic study. Virchows Arch [Cell Pathol] 45:221–240
Newton DL, Henderson WR, Sporn MB (1980) Structure-activity relationships of retinoids in hamster tracheal organ culture. Cancer Res 40:3413–3425
Puts JJG, Moesker O, Kenemans P, Vooijs GP, Ramaekers FCS (1985) Expression of cytokeratins in early neoplastic epithelial lesions of the uterine cervix. Int J Gynecol Pathol 4:300–313
Ramaekers FCS, Puts JJG, Moesker O, Kant A, Huysmans A, Haag D, Jap PHK, Herman CJ, Vooijs GP (1983) Antibodies to intermediate filament proteins in the immunohistochemical identification of human tumours: An overview. Histochem J 15:691–713
Ramaekers F, Huijsmans A, Schaart G, Moesker O, Vooijs G (1987) Tissue distribution of keratin 7 as monitored by a monoclonal antibody. Exp Cell Res 170:235–249
Rutten AAJJL, Wilmer JWGM (1986) Effect of cigarette smoke condensate and norharman on the induction of SCEs by direct and indirect mutagens in CHO cells. Mutat Res 172:61–67
Rutten AAJJL, Beems RB, Wilmer JWGM (1988 a) Effects of all-trans retinol on hamster tracheal epithelium in organ culture. I. A cell proliferation study. Virchows Arch [Cell Pathol] 55:167–175
Rutten AAJJL, Beems RB, Wilmer JWGM (1988b) Effects of all-trans retinol on hamster tracheal epithelium in organ culture. II. A histomorphological study. Virchows Arch [Cell Pathol] 55:177–186
Rutten AAJJL, Jonger WMF, de Haan LHJ, Hendriksen EGJ, Koeman JH (1988c) Effect of retinol and cigarette smoke condensate on dye-coupled intercellular communication between hamster tracheal epithelial cells in serum-free medium. Carcinogenesis 9:315–320
Rutten AAJJL, Bruyntjes JP, Ramaekers FCS (1988d) Intermediate filament expression in normal and vitamin A-deprived cultured hamster tracheal epithelium as detected by monoclonal antibodies. A study with emphasis on histomorphological changes. Virchows Archiv B [Cell Pathol] 56:103–110
Sigler RE, Jones RT, Hebel JR, McDowell EM (1987) Hamster tracheal organ culture in serum-free media: A quantitative comparison of in vitro epithelial morphology with that of in vivo controls. In Vitro 23:100–110
Sigler RE, Newkirk C, McDowell EM (1988) Histogenesis and morphogenesis of epidermoid metaplasia in hamster tracheal organ expiant culture. Virchows Arch [Cell Pathol] 55:47–55
Sporn MB, Dunlop NM, Newton DL, Henderson WR (1976) Relationships between structure and activity of retinoids. Nature 263:110–113
Sporn MB, Roberts AB (1984) Biological methods of analysis and assay or retinoids relationships between structure and activity. In: Sporn, MB, Roberts AB, Goodman DS (eds) The retinoids, vol 1. Academic Press, Inc, Orlando, FL, pp 236–275
Van DuurenBL, Sivak A, Segal A, Orris L, Langseth L (1966) The tumorpromoting agents of tobacco leaf and tobacco smoke condensate. JNCI 37:519–526
Wynder EL, Hoffmann D (1961) A study of tobacco carcinogenesis. VIII. The role of the acidic fractions as promoters. Cancer 14:1306–1315
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Rutten, A.A.J.J.L., Bruyntjes, J.P. & Ramaekers, F.C.S. Effect of cigarette smoke condensate and vitamin A depletion on keratin expression patterns in cultured hamster tracheal epithelium. Virchows Archiv B Cell Pathol 56, 111–117 (1988). https://doi.org/10.1007/BF02890009
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DOI: https://doi.org/10.1007/BF02890009