Abstract
According to the definition of cytokine, the direction of signaling should be from cytokine to receptor. The counter receptor was presented. Membrane bound macrophage colony-stimulating factor (m-M-CSF) and its receptor (M-CSF-R) were shown in human leukemic cell line J6-1 as autojuxtacrine mechanism. Soluble M-CSF receptor (sM-CSF-R), which was isolated from J6-1 cells membrane, was added into J6-1 cell culture. It was observed inhibition of J6-1 cell proliferation, decreasing of mitosis index and ratio of multinuclear cells, enlargement of cell diameter and volume, down regulation of numerous surface antigens. Dramatic change of intracellular pH was shown between several min to 20 min after treatment of sM-CSF-R. It suggested that some information was transmitted via m-M-CSF from sM-CSF-R. This counter signaling was not influenced by saccharification of m-MCSF.
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References
Anklesaria, P., Teixido, J., Laiho, M.et al., Cell-cell adhesion mediated by binding of membrane-anchored transforming growth factor and epidermal growth factor receptor promotes cell proliferation,Proc. Natl. Acad. Sci. USA, 1990, 87: 3289.
Wu, K. F., Zhang, Y. Z., Qi, S. L.et al., Establishment of myeloid, monocytic leukemic cell lines and studies of their cell biological properties,Acta Genetica Sinica (in Chinese), 1980, 7: 136.
Wu, K. F., Rao, Q., Zheng, G. G.et al., Enhancement of J6-1 human leukemic cell proliferation by cell-cell contact: Role of an M-CSF-like membrane-associate growth factor MAF-J6-1,Leukemia Res., 1994, 18: 843.
Wu, K. F., Zheng, G. G., Geng, Y. Q.et al., Auto-juxtacrine mediated via m-M-CSF/M-CSF-R on human leukemic cells,Cell Res., 1995(suppl): 69.
Wu, K. F., Rao, Q., Zheng, G. G.et al., Enhancement of J6-1 human leukemic cell proliferation by MAF-J6-1 through a cell-cell contact mechanism: II. Role of an M-CSf receptor-like membrane protein,Leukemia Res., in press.
Geng, Y. Q., Rao, Q., Kong, J.et al., Preparation and characterization of McAb of leukemic cell membrane associated factor (MAF-J6-1),Chinese J. Hematology (in Chinese), 1994, 15(12): 630.
Song, Y. H., Yang, C. L., Qi, S. L.et al., Studies on micromegakaryocytes in myelodysplatic syndromes (MDS),Proc. CAMS & PUMC, 1988, 3: 33.
Wang, X., Cai, H. G., Chen, P. Z.et al., Amplification of c-fms extracellular domain from human placenta,Chinese Biochemical Journal (in Chinese), 1997, in press.
Uemura, N., Binding of membrane-anchored macrophage colony-stimulating factor (M-CSF) to its receptor mediates specific adhesion between stromal cells and M-CSF receptor-bearing hematopoietic cells,Blood, 1993, 82: 2634.
Weiser, P., Endosomal targeting by the cytoplasmic tail of membrane immunoglobulin,Science, 1997, 276: 407.
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Kefu, W., Zhihong, H., Guoguang, Z. et al. Receptor role of membrane bound macrophage colony-stimulating factor. Chin. Sci. Bull. 43, 1034–1037 (1998). https://doi.org/10.1007/BF02884643
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DOI: https://doi.org/10.1007/BF02884643