Abstract
We have developed a biostrip for determination of urea in serum. The test strip is based on enzymatic assay where urease has been immobilized on the chromatographic paper along with chromogen, phenol red. The chromogen is easily soluble in water and does not require other components for the color change. Serum urea reacts with urease and water to liberate ammonia and carbon dioxide. The liberated ammonia changes the pH of the reaction medium, which is monitored by the chromogen phenol red. A single step working reagent strip has been developed and the reaction is completed within 50 seconds at room temperature. With this test strip urea concentration is measured in serum as low as 0.15 g/L. The speed and convenience of determining urea in serum by this strip instantly makes it well suited for individuals, physicians and emergency centres.
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References
Court, J.M., Davis, H.E. and Ferguson, R. (1972) Diastix and Keto-diastix-A new semiquantitative test for glucose in urine. Med. J. Aust. 1, 525–528.
Assadi, F.K. and Fornell, L. (1986) Estimation of urine specific gravity in nanotes with a reagent strips. J. Pediatr. 108, 995–996.
Hurlburt, T.A. and Littenberg, B. (1991) The diagnostic accuracy of rapid dipstick test to predict urinary tract infection. Amer. J. Clin. Pathol. 96(5), 582–590.
Jain, A.V., Ross, P.F. and Carlso, M.P. (1999) Screening nitrate in foragen with a test strip: Collaborative study. J AOAC Int. 82(1), 9–15.
Marshall, S.M., Shearing, P.A. and Albert, K.G. (1992) Micral test strips evaluated for screening of albuminemia. Clin. Chem. 38, 588–591.
Orsonneau, J.L., Massoubre, C., Cabanes, M. and Lustenberge P. (1992) Simple and sensitive determination of urea in serum and urine. Clin. Chem. 38, 619–623.
Naslund, B., Sthale, L., Lundin, A., Anderstam, B., Arner, P. and Bergstrom J. (1998) Luminometric single step urea assay using ATP-hydrolyzing urease. Clin. Chem. 44, 1964–1973.
Tarnoky, A.L. (1964) Screening method for blood urea estimation. Clin. Chem. Acta. 10, 253–255.
Li, X, (1993) Application and preparation of a BUN test strip, Chung-Kuo-I-Hsueh-Yuan-Hsueh-Pao, (Chinese J.) 15(2), 152–154.
Rogge, J.D., Wagner, D.R., Carrico, R.J., Glowinski, E.A., Mahoney, S.J., Bogusiaski, R.C. and Genta, R.M. (1995) Evaluation of a new urease reagent strip for detection ofHelicobacter pylori in gastric biopsy specimen. Amer. J. Gastroenterol. 90(11), 1965–1967.
Lequang, N.T., Migueres, G., Roche, D., Pelladeau, M.L. and Labrousse, F. (1987) Improved dye procedure for determining urea concentration by using o-phthalaldehyde and naphthylethylenediamine. Clin. Chem. 33, 192.
Lespinas, F., Dupuy, D., Revol, F. and Aurby, C. (1989) Enzymatic urea assay: A new colorimetric method based on hydrogen peroxide measurement. Clin. Chem. 35(4), 654–658.
Waybenga, D.R., Giorgrio, J.D. and Pileggi, V.J. (1971) Mannual automated methods for urea nitrogen measurement in whole serum. Clin. Chem. 17, 891–895.
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Kumar, H., Kumar, A., Kumari, P. et al. A test strip for the estimation of urea in serum. Indian J Clin Biochem 15, 124–127 (2000). https://doi.org/10.1007/BF02883740
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DOI: https://doi.org/10.1007/BF02883740