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A Dual Electrochemical Sensor Based on a Test-strip Assay for the Quantitative Determination of Albumin and Creatinine

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Abstract

A dual-electrochemical sensor based on a test-strip assay with immunochemistry and enzyme reactions has been developed for the determination of albumin and creatinine. Each nitrocellulose membrane with an immobilization area of an anti-albumin antibody or three enzymes was prepared in the device with three working electrodes for measuring albumin, creatinine, and ascorbic acid, as well as an Ag/AgCl electrode used as a counter/pseudo-reference electrode. The reactions of three enzymes were initiated by flowing a solution containing creatinine to detect an oxidation current of hydrogen peroxide. A sandwich-type immunocomplex was formed by albumin and antibody labeled with glucose oxidase (GOx). Captured GOx catalyzed the reduction of Fe(CN)63- to Fe(CN)64-, which was oxidized electrochemically to determine the captured albumin. The responses for creatinine and albumin increased with the concentrations in millimolar order and over the range 18.75–150 μg mL−1, respectively. The present sensor would be a distinct demonstration for producing quantitative dual-assays for various biomolecules used for clinical diagnoses.

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Acknowledgments

This work was partly supported by JSPS KAKENHI Grant No. 26288071.

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Authors and Affiliations

  1. University of Hyogo, 3-2-1 Kouto, Kamigori, Ako, Hyogo, 678-1297, Japan

    Tomoyuki Yasukawa, Yuya Kiba & Fumio Mizutani

Authors
  1. Tomoyuki Yasukawa
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  2. Yuya Kiba
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  3. Fumio Mizutani
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Correspondence to Tomoyuki Yasukawa.

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Yasukawa, T., Kiba, Y. & Mizutani, F. A Dual Electrochemical Sensor Based on a Test-strip Assay for the Quantitative Determination of Albumin and Creatinine. ANAL. SCI. 31, 583–589 (2015). https://doi.org/10.2116/analsci.31.583

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  • DOI: https://doi.org/10.2116/analsci.31.583

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