The course of root differentiation from root primordia in poplar stems

Abstract

Upon rooting of poplar stem cuttiags the total inductive stimulation does not take place, but the individual root primordia maintain a relative independence. This becomes evident by various time parameters of their differentiation. Under experimental conditions, in dormant cuttings from one year individuals of the hybrid I 214 the activation of the first root primordia occurs after 24 h, the pre-emergent development of the roots formed was completed after 72 h.

The activated root primordium is divided into two regions. In the distal region with the predominating cell division the root apex with histogens is formed by the action of initials. From peripheral cell layers of the distal region the so-called “Wurzeltasche” develops which covers the root cap. Due to cell elongation in the proximal region the root apex is pushed up towards the stem surface. The beginning differentiation of the connective vascular tissue is a preparative step for the connection of the vascular system of the developing root with the secondary vascular system of the maternal stem. Following the penetration of the root through the peripheral stem tissues this connection is realized with progressing development. In the developed root the protoxylem elements differentiate continuously and acropetally in direct continuity with tracheids of the basal connective region.