Abstract
N-methyl-N-nitroso-N′-nitroguanidine was used for the induction of two types of mutations in the PN strain ofMycobacterium phlei. Nineteen auxotrophic, 136 scotochromogenic and 50 achromogenic mutants were isolated after of treatment with nitrosoguanidine at a concentration of 1,000 μg/ml. Auxotrophic mutants required primarily amino acids and vitamins and half of them may be used for further genetic work due to their low frequency of spontaneous reversions. Colonies of scotochromogenic mutants were orange with the exception of one, which was strawberry red. Most scotochromogenic mutants were detected on a streptomycin containing medium. Roughly two thirds of the scotochromogenic mutants and one half of achromogenic mutants did not revert to the original photochromogenic character.
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References
Adelberg, E. A., Mandel, M., Chein Ching Chen, G.:Optimal conditions for mutagenesis by N-methyl-N′-nitro-N-nitrosoguanidine in Escherichia coli K 12. Biochem. biophys. Res. Commun. 18: 788, 1965.
McCalla, D. R.:Reaction of N-methyl-N′-nitro-N-nitrosoguanidine and N-methyl-N-nitroso-p-toluenesulfonamide with DNA in vitro. Biochim. biophys. Acta 155: 114, 1968.
Cerdá-Olmedo, E., Hanawalt, P. C.:Macromolecular action of nitrosoguanidine in Escherichia coli. Biochim. biophys. Acta 142: 450, 1967a.
Cerdá-Olmedo, E., Hanawalt, P. C.:Repair of DNA damaged by N-methyl-N′-nitro-N-nitrosoguanidine in Escherichia coli. Mutation Res. 4: 369, 1967b.
Craddock, V. M.:The reaction of N-methyl-N′-nitro-N-nitrosoguanidine with deoxyribonucleic acid. Biochem. J. 106: 921, 1968.
Eisenstark, A., Eisenstark, R., Van Sickle, R.:Mutation of Salmonella typhimurium by nitrosoguanidine. Mutation Res. 2: 1, 1965.
Hubáček, J., Málek, I.:Einige biologische und biochemische Eigenschaften säurefester und nichtsäurefester Stämme des Mycobacterium phlei. Fol. biol. 4: 220, 1958.
Koníček, J., Málek, I.:The induction of pigmentation change in a non-acid-fast strain of Mycobacterium phlei by ultraviolet radiation. Fol. microbiol. 12: 417, 1967.
Koníček, J., Málek, I.:The mutagenic effect of ethyl methanesulphonate on a non-acid-fast strain of Mycobacterium phlei. Fol. microbiol. 14: 194, 1969.
Mandel, J. D., Greenberg, J.:A new chemical for bacteria, 1-methyl-3-nitro-1-nitrosoguanidine. Biochem. biophys. Res. Commun. 3: 575, 1960.
Megnet, R.:Screening of auxotrophic mutants of Schizosaccharomyces pombe with 2-deoxyglucose. Mutation Res. 2: 328, 1965.
Nordström, K.:Induction of the petite mutation in Saccharomyces cerevisie by N-methyl-N′-nitro-N-nitrosoguanidine. J. gen. Microbiol. 48: 277, 1967.
Tarshis, M. S.:Preliminary observations on the development of atypical (chromogenic) variants of the H 37 Rv strain of Mycobacterium tuberculosis under the influence of streptomycin and isoniazid in vitro. Am. Rev. Tuberc. 78: 921, 1958.
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Koníček, J., Málek, I. The use of nitrosoguanidine in the study of mutagenesis in a non-acid-fast strain ofMycobacterium phlei . Folia Microbiol 15, 82–87 (1970). https://doi.org/10.1007/BF02880089
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DOI: https://doi.org/10.1007/BF02880089