Abstract
Five scotochromogenic mutants and 11 achromogenic mutants were induced by UV irradiation of the non-acid-fast photochromogenic PN strain ofMycobacterium phlei. Spontaneous scotochromogenic and achromogenic mutants were not obtained. Colonies of the scotochromogenic mutants are orange, except for one mutant which is ochre. Three mutants are resistant to STM. Out of 11 achromogenic mutants 3 were induced by UV treatment of the original photochromogenic strain, 8 were prepared from the scotochromogenic mutant. No significant differences in the sensitivity to UV rays were found among the scotochromogenic mutant, achromogenic mutant and the photochromogenic PN strain ofMycobacterium phlei under the given experimental conditions. Scotochromogenic mutants and most achromogenic mutants are stable and suitable for further genetic investigation. Pigmentation changes can be used as genetic marker in mutation studies.
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Goodwin, T. W.:The comparative biochemistry of the carotenoids. Ed. Chapman & Hall, London, 1952.
Hubáček, J., Málek, I.:Einige biologische und biochemische Eigenschaften säurefester und nichtsäurefester Stämme des Mycobacterium phlei. Fol. microbiol. 4: 220, 1958.
Jensen, S. L.:Biosynthesis and function of carotenoid pigments in microorganisms. Ann. Rev. Microbiol. 19: 163, 1965.
Penso, G., Ortali, V., Gaudiano, A., Princivalle, M., Vella, L., Zampieri, A.:Studi e ricerche sui micobatteri VII. IL Mycobacterium phlei. Estratto dai rendiconti Dell’ Istituto superiore di Sanita. XIV: 855, 1951.
Radochová, M., Koníček, J., Málek, I.:The mutagenic effect of ultraviolet radiation and nitrous acid on Mycobacterium phlei. Fol. microbiol. 11: 163, 11: 163, 1966.
Tarshis, M. S.:Preliminary observations on the development of atypical (chromogenic) variants of the H 37 Rv strain of Mycobacterium tuberculosis under the influence of streptomycin and isoniazid in vitro. Am. Rev. Tuberc. 78: 921, 1958.
Tsukamura, S.:Biological significance of pigments of Mycobacteria I and II. Japan. J. Tuberc. 12: 1, 1963a.
Tsukamura, S.:Biological significance of pigments of Mycobacteria III. Japan. J. Tuberc. 12: 7, 1963b.
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Koníček, J., Málek, I. The induction of pigmentation change in a non-acid-fast strain ofMycobacterium phlei by ultraviolet radiation. Folia Microbiol 12, 417–421 (1967). https://doi.org/10.1007/BF02864947
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DOI: https://doi.org/10.1007/BF02864947