Abstract
Hybrid plasmid pIM138 was constructed by insertion of a chromosomal fragment with the threonme operon fromEscherichia coli into the pBR322 vector. Molar mass of pIM138 was 2.8 Mg/mol. Heteroduplexes between pBR322 vector and pIM138 hybrid DNA molecules were prepared. The hybrid plasmid shows a high stability against the curing effect of rifampicin and clorobiocm inE. coli SK1590thr host.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Adelberg E. A., Mandel M., Chein Ching Chen G.. Optimal conditions for mutagenesis by N methyl N nitro N nitiosoguanidine inEscherichia coli K12.Biochem Biophys Res Commun.18 788 (1965)
Birnboim H. C., Doly J.: A rapid alkaline extraction procedure for screening recombinant plasmid DNA.Nucleic Acid Res.7, 1513 (1975)
Brana H., Benada O., Kosych V. G., Hubaček J.: Physical characterization of the plasmid pON5300.Molec Gen Genet 184, 107 (1981)
Brana H., Benada O., Navratil O., Čejka K., Hubaček J.: Characteristic of the hybrid plasmid pIM138, inPlasmids and Antibiotic Resistance (Mitsuhashi S., Rosival L., Krcmery V., Eds) Avicenum Medical Press, Prague (in press)
Cossart P., Katinka M., Yaniv M., Girons I. S., Cohen G. N., Construction and expression of a hybrid plasmid containing theEschenchia coll thrA and thrB genesMolec Gen Genet 175, 39 (1979)
Čejka K., Holubova I., Hubaček J.: Curing effect of clorobiocin onEschenchia coli plasmidsMolec Gen Genet.186, 153 (1982)
Dagert M., Ehrlich S. D., Prolonged incubation in calcium chloride improves the competenceof Eschenchia coli cells.Gene.6, 23 (1979)
Davis R.W., Simon M., Davidson N.: Electron microscope heteroduplex methods for mapping regions of base sequence homology m nucleic acids, p. 413 inMethods in Enzymology, Vol. 21, Nucleic Acids, Part D (Grossman L., Moldave K., Eds). Academic Press, New York-London 1971.
Debabov V.G.: Gene engineering and microbiological industry, p. 345 inOverproduction of Microbial Products, FEMS Symp. No. 13 (V. Krumphanzl, B. Sikyta, Z. Vaněk, Eds). Aca demic Press, London 1982.
Eckhardt T.: A rapid method for the identification of plasmid deoxyribonucleic acid m bac teria.Plasmid 1, 584 (1978).
Fraley R. T., Fornani O.S., Kaplan S.: Entrapment of a bacterial plasmid m phosphohpid vesicles: Potential for gene transfer.Proc.Nat.Acad.Sci. 76, 3348 (1979).
Garaev M.M., Bobkov A.F., Bobkova A.F., Kalinin V.N., Smirnov V.D., Khudakov Yu.E., Tichonenko T.I.: The site-specific deletion m plasmid pBR322.Gene 18, 21 (1982).
Gardner J.F.: Regulation of the threonine operon: Tandem threonme and isoleucine codons in the control region and translational control of transcription termination.Proc.Nat.Acad. Sci. 76, 1706 (1979).
Greene P.J., Heyneker H.L., Bolivar F., Rodriquez R.L., Betlach M.C., Covarrubias A.A., Backman K., Russek D.J., Tait R., Boyer H.W.: A general method for the purification of restriction enzymes.Nucleic Acid Res.5, 2373 (1978).
Hansen B., Olsen R.H.: Isolation of large bacterial plasmids and characterization of the P2 incompatibility group plasmids pMGl and pMG5.J.Bacteriol. 135, 227 (1978).
Hubáček J., Kosych V.G.: Trans-dominant mutation affecting restriction and modification inEscherichia coli K12.Molec.Gen.Genet. 187, 459 (1982).
Keller E.B., Calvo J.M.: Alternative secondary structure of leader RNAs and the regulation of thetrp, phe, his, thr andleu operons.Proc.Nat.Acad.Sci. 76, 6186 (1979).
Knopf K.W.: Simple isolation method and assay for T4 DNA ligase and characterization of the purified enzyme.Eur. J.Biochem. 73, 33 (1977).
Kozlov Yu.I., Kochetova L.P., Livshits V.A., Mashko S.V., Moshentseva V.N., Rebentish B.A., Rozinov M.N., Khurges E.M., Yankovsky N.K., Debabov V.G.: Cloning of the threonme operon genes inEscherichia coli.Genetika 16, 66 (1980).
Kozlov Yu.I., Mashko S.V., Savelyev V.I., Bodush V.G., Moshentseva V.N., Permogorov V.I., Debabov V.G.: Interaction of RNA polymerase with hybrid plasmids carryingEsche richia coli threonme operon genes.Molekul.Biologija 15, 1245 (1981).
Kushner R.S.: An improved methods for transformation ofEscherichia coli with ColE1 derived plasmids p. 17 inGenetic Engineering (H.W. Boyer, S. Nicosia, Eds). Elsevier, Amsterdam 1978.
Sharp P.A., Hsu T.M., Ohtsubo E., Davidson N.: Electron microscope heteroduplex studies of sequence relations among plasmids ofEscherichia coll. I. Structure of F′ prime factors.J. Mol.Biol. 71, 471 (1972).
Sucliffe J.C.: pBR322 restriction map derived from the DNA sequence: Accurate DNA size markers up to 4361 nucleotide pairs long.Nucleic Acid Res.5, 2721 (1978).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Braná, H., Benada, O., Navrátil, O. et al. Stability of the hybrid plasmid pIM138 and its curing by some eliminating agents. Folia Microbiol 28, 441–445 (1983). https://doi.org/10.1007/BF02879679
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02879679