Abstract
Wild-type and mutant (glucosephosphate isomerase, pyruvate kinase and respiratory deficientrho) strains were used to determine the kinetics of substrate-induced H+ efflux in dilute suspensions, glucose-induced production of titratable acidity in intact cells and cell-free extracts, and kinetics of extracellular titratable acidity production (pH-stat). The results indicate that (1) initial phases of H+ efflux proceed at the expense of preexisting cell acidity reserves while subsequent efflux is supported by de novo formed acidity, (2) apart from regulation by pHout the H+ efflux is subject to intracellular control, (3) intracellular acidity level is controlled separately from H+ efflux. Tentative scheme is proposed for the regulation of H+ fluxes inS. cerevisiae.
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Sigler, K., Pascual, C. & Romay, C. Intracellular control of proton extrusion inSaccharomyces cerevisiae . Folia Microbiol 28, 363–370 (1983). https://doi.org/10.1007/BF02879485
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DOI: https://doi.org/10.1007/BF02879485