Skip to main content
SpringerLink
Log in

Inhibition of succinate production during yeast fermentation by deenergization of the plasma membrane

  • Published:
Current Microbiology Aims and scope Submit manuscript

Abstract

Succinate production by a respiratory-deficient yeast was inibited by substances known to depolarize the plasma membrane. These substances include high concentrations of the permeable cation potassium, the ATPase inhibitor diethylstilbestrol, the polyene antibiotic amphotericin B, and the uncouplers carbonyl cyanidem-chlorophenylhydrazone and dinitrophenol. Results suggest that succinate effux from yeast cells is driven by membrane energization in the form of an electrical potential. As sucinate is one of the major by-products of alcoholic fermentation, deenergization of yeast plasma membrane may be a useful approach to increasing the yield of ethanol in industrial fermentations.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Literature Cited

  1. Cirillo, V. P., Harsch, M., Lampen, J. O. 1964. Action of the polyene antibiotics filipin, nystain andn-acetylcandidin on the yeast cell membrane. Journal of General Microbiology35:249–259.

    PubMed  Google Scholar 

  2. Eddy, A. A. 1978. Proton-dependent solute transport in microorganisms. Current Topics in Membranes and Transport10:279–360.

    Google Scholar 

  3. Freitas Valle, A. B., Panek, A. D., Mattoon, J. R. 1978. Colorimetric determination of succinic acid using yeast succinate dehydrogenase. Analytical Biochemistry91:583–599.

    PubMed  Google Scholar 

  4. Hauer, R., Hofer, M. 1978. Evidence for interaction between the energy-dependent transport of sugars and the membrane potential in the yeastRhodotorula gracilis. Journal of Membrane Biology43:335–349.

    Google Scholar 

  5. Heerde, E., Radler, F. 1978. Mechanism of the anaerobic formation of succinic acid bySaccharomyces cerevisiae. Archives of Microbiology117:269–276.

    Google Scholar 

  6. Kovac, L. 1974. Biochemical mutants: An approach to mitochondrial energy coupling. Biochimica et Biophysica Acta346:101–135.

    PubMed  Google Scholar 

  7. Lagunas, R., Gancedo, J. M. 1973. Reduced pyridine-nucleotides balance in glucose-growingSaccharomyces cerevisiae. European Journal of Biochemistry37:90–94.

    PubMed  Google Scholar 

  8. Oura, E. 1977. Reaction, products of yeast fermentaions. Process Biochemistry12:19–21.

    Google Scholar 

  9. Palacios, J., Serrano, R. 1978. Proton permeabiliy induced by polyene antibiotics. A plausible mechanism for their inhibition of maltose fermentation in yeast. FEBS Letters91:198–201.

    PubMed  Google Scholar 

  10. Peña, A. 1975. Studies on the mechanism of K transport in yeast. Archives of Biochemistry and Biophysics167:397–409

    PubMed  Google Scholar 

  11. Raven, J. A., Smith, F. A. 1976. The evolution of chemiosmotic energy coupling. Journal of Theoretical Biology57:301–312.

    PubMed  Google Scholar 

  12. Serrano, R. 1977. Energy requirements for maltose transport in yeast. European Journal of Biochemistry80:97–102.

    PubMed  Google Scholar 

  13. Serrano, R. 1978. Characterization of the plasma membrane ATPase ofSaccharomyces cerevisiae. Molecular and Cellular Biochemistry22:51–63.

    PubMed  Google Scholar 

  14. Serrano, R. 1980. Effect of ATPase inhibitiors on the proton pump of respiratory-deficient yeast. European Journal of Biochemistry105:419–424.

    PubMed  Google Scholar 

  15. Slayman, C. L., Long, W. S., Lu, C. Y. H. 1973. The relationship between ATP and an electrogenic pump in the plasma membrane ofNeurospora crassa. Journal of Membrane Biology14:305–338.

    PubMed  Google Scholar 

  16. Stein, W. D. 1967. The movement of molecules across cell membranes. New York: Academic Press.

    Google Scholar 

  17. Ten Brink, B., Konings, W. N. 1980. Generation of an electrochemical proton gradient by lactate effux in membrane vesicles ofEscherichia coli. European Journal of Biochemistry111:59–66.

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Instituto of Enzimología y Patología Molecular del C.S.I.C., Facultad de Medicina de la Univeridad Autónoma, Marid-34, Spain

    Antonio F. Duro & Ramón Serrano

Authors
  1. Antonio F. Duro
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ramón Serrano
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Duro, A.F., Serrano, R. Inhibition of succinate production during yeast fermentation by deenergization of the plasma membrane. Current Microbiology 6, 111–113 (1981). https://doi.org/10.1007/BF01569014

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01569014

Keywords

Search

Navigation