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Development and characterization of an adapted form ofdroopy, a diploid potato mutant deficient in abscisic acid

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Abstract

A cultivated diploid potato breeding population has been selected for adaptation to growing, tuberizing (under relatively long days), and storing (including long dormancy) under New Brunswick conditions. In this population, a mutant was discovered that appeared similar to the earlier describeddroopy mutant, which is deficient in abscisic acid and is unable to regulate water loss from its leaves. The physiology and genetics of the newly discovered mutant were studied and compared in detail with the description ofdroopy. This mutant has a longer tuber dormancy than the originaldroopy. In families segregating fordroopy and normal, similar dormancies and endogenous abscisic acid levels in tubers were observed betweendroopy and normal genotypes. The effect of the mutant gene appears to be tissue specific, affecting aboveground plant parts only. A test for allelism indicated that this mutant is allelic todroopy. Classical linkage analyses confirmed previously reported close linkage between theDr (droopy) and theS (incompatibility) loci. TheDr locus has been mapped in this study to the top of chromosome I. Several test crosses indicated reciprocal differences in the segregation ratios betweendroopy and normal In keeping with thedroopy (drdr) genotype, drought-stressed leaves of the mutant were incapable of increasing abscisic acid production compared to the normal. This mutant, with its apparent developmentally restricted expression, may be useful in elucidating the genetic and physiological processes associated with such major events as tuberization, response to drought stress and tuber dormancy.

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Authors and Affiliations

  1. Agriculture and Agri-Food Canada, Potato Research Centre, P.O.Box 20280, E3B 4Z7, Fredericton, NB, Canada

    H. De Jong, W. K. Coleman, L. Russell, V. J. Burns & E. Tremblay-Deveau

  2. Agriculture and Agri-Food Canada, Lethbridge Research Centre, P.O.Box 3000, T1J 4B1, Lethbridge, AB, Canada

    L. M. Kawchuk & C. A. Verhaeghe

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  1. H. De Jong
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  2. L. M. Kawchuk
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  3. W. K. Coleman
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  4. C. A. Verhaeghe
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  5. L. Russell
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  6. V. J. Burns
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  7. E. Tremblay-Deveau
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Correspondence to H. De Jong.

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Jong, H.D., Kawchuk, L.M., Coleman, W.K. et al. Development and characterization of an adapted form ofdroopy, a diploid potato mutant deficient in abscisic acid. Amer J of Potato Res 78, 279–290 (2001). https://doi.org/10.1007/BF02875693

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